relation: http://oar.icrisat.org/9122/ title: Acetylene reductase activity and molecular characterization of plant growth promoting rhizobacteria to know efficacy in integrated nutrient management system creator: Kaushal, M creator: Kaushal, R subject: Food and Nutrition subject: Agriculture-Farming, Production, Technology, Economics description: Isolates from rhizosphere were screened and characterized for their plant growth promotion and biocontrol properties as per standard methods. Five isolates (MK2, MK4, MK5, MK7 & MK9) showed the maximum plant growth promoting attributes like P-solubilization, N-fixation etc. Acetylene reduction assay (ARA) method was used to study N2 fixing ability. The bacterial isolate MK5 fixed the maximum nitrogen, i.e., 437.26 ηmole C2H4 h-1 mg-1 protein (109 mg of N2 fixed/ha/d). The isolates MK5 and MK7 showed the maximum homology with Bacillus pumilus and B. subtilis, respectively by 16S rRNA analysis. The bacterial isolate MK5 in conjunction with 75% level of recommended dose of N and P fertilizers gave increase yield of cauliflower crop by 27.29% over recommended doses of NPK with a saving of 25% N and 25% P fertilizers. publisher: NISCAIR date: 2015 type: Article type: PeerReviewed format: application/pdf language: en identifier: http://oar.icrisat.org/9122/1/IJB_14_221-227_2015.pdf identifier: Kaushal, M and Kaushal, R (2015) Acetylene reductase activity and molecular characterization of plant growth promoting rhizobacteria to know efficacy in integrated nutrient management system. Indian Journal of Biotechnology, 14 (2). pp. 221-227. ISSN 0975-0967