@article{icrisat9122,
            year = {2015},
           title = {Acetylene reductase activity and molecular characterization of plant growth promoting rhizobacteria to know efficacy in integrated nutrient management system},
          volume = {14},
          number = {2},
           pages = {221--227},
          author = {M Kaushal and R Kaushal},
       publisher = {NISCAIR},
         journal = {Indian Journal of Biotechnology},
             url = {http://oar.icrisat.org/9122/},
        abstract = {Isolates from rhizosphere were screened and characterized for their plant growth promotion and biocontrol properties as per standard methods. Five isolates (MK2, MK4, MK5, MK7 \& MK9) showed the maximum plant growth promoting attributes like P-solubilization, N-fixation etc. Acetylene reduction assay (ARA) method was used to study N2 fixing ability. The bacterial isolate MK5 fixed the maximum nitrogen, i.e., 437.26 {\ensuremath{\eta}}mole C2H4 h-1 mg-1 protein (109 mg of N2 fixed/ha/d). The isolates MK5 and MK7 showed the maximum homology with Bacillus pumilus and B. subtilis, respectively by 16S rRNA analysis. The bacterial isolate MK5 in conjunction with 75\% level of recommended dose of N and P fertilizers gave increase yield of cauliflower crop by 27.29\% over recommended doses of NPK with a saving of 25\% N and 25\% P fertilizers.},
        keywords = {Acetylene reduction assay (ARA), Bacillus spp., Biocontrol, Cauliflower, INM, PGPR, 16S rRNA}
}