OAR@ICRISATGenetic transformation studies on sorghum (Sorghum bicolor) for sucrose isomeraseM JShyam sunderauthorSeeds of sweet sorghum cultivars SSV74 and SSV84 were the primary explants used for the introduction pdSI genes(expression of Sucrose Isomerase enzyme) which converts into Isomaltulose(palatinose,α-D-glycopyranosyl-1,6-Dfructofuranose). Isomaltulose is an isomer of sucrose which has greater health advantages for diabetics and used as low calories sweetener, it is also a renewable starting material in industrial productions. The pdSI gene was under regulation of 35S promoter cloned into binary vector PMDC99. Both the vectors also contain hygromycin phosphotransferase gene (hptII) as plant selection marker against hygromycin A kill-curve experiment was performed to determine the optimal concentration at which the control explants is able to survive rate was high in 2.5 & 0.5 mg/L concentration whereas 7.5 mg/L, the survival rate of approximately 12-15%. Agrobacterium cloned with pdSI gene was used for transfer of the genes into sweet sorghum cultivar. Pre-soaked imbibed seeds of sweet sorghum were punctured using sterilized needle and dipped in Agro culture (with pdSI gene) in YEB media for 15-20 minutes. The seeds were co-cultivated on MS media 48-72 hours. The explants were then transferred in to MS media augmented with cefotaxime and hygromycin. The selection marker strategy is as follows, During germination stage hygromycin concentration was 2.5mg/L, in elongation stage,5mg/L and rooting was done at 7.5mg/L. plantlets which are showed rooting and survival at selection pressure of 7.5mg/L were then transferred to soil for acclimatization. Leaf sample of healthy, putative plants were collected and DNA was extracted using CTAB method for further molecular characterization.Sorghum2013Jawaharlal Nehru Technological University;Centre for Biotechnology Institute of Science and TechnologyThesis

For work being deposited by its own author: In self-archiving this collection of files and associated bibliographic metadata, I grant OAR@ICRISAT the right to store them and to make them permanently available publicly for free on-line. I declare that this material is my own intellectual property and I understand that OAR@ICRISAT does not assume any responsibility if there is any breach of copyright in distributing these files or metadata. (All authors are urged to prominently assert their copyright on the title page of their work.)

For work being deposited by someone other than its author: I hereby declare that the collection of files and associated bibliographic metadata that I am archiving at OAR@ICRISAT) is in the public domain. If this is not the case, I accept full responsibility for any breach of copyright that distributing these files or metadata may entail.

Clicking on the deposit button indicates your agreement to these terms.