eprintid: 9112
rev_number: 12
eprint_status: archive
userid: 17
dir: disk0/00/00/91/12
datestamp: 2015-11-04 03:45:42
lastmod: 2015-11-04 03:45:42
status_changed: 2015-11-04 03:45:42
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
creators_name: Moumouni, K H
creators_name: Kountche, B A
creators_name: Jean, M
creators_name: Hash, C T
creators_name: Vigouroux, Y
creators_name: Haussmann, B I G
creators_name: Belzile, F
icrisatcreators_name: Hash, C T
affiliation: ICRISAT (Niamey)
affiliation: Institut de Recherche pour le Développement (Montpellier)
affiliation: Université Abdou Moumouni (Niamey)
affiliation: University of Hohenheim (Stuttgart)
country: Canada
country: Niger
country: France
country: Germany
title: Construction of a genetic map for pearl millet, Pennisetum glaucum (L.) R. Br., using a genotyping-by-sequencing (GBS) approach
ispublished: pub
subjects: S1.5
subjects: s2.13
divisions: D4
crps: crp1.4
full_text_status: restricted
keywords: Pearl millet, Genetic map, SNP, Genotyping-by-sequencing
abstract: Pearl millet is the main component of traditional farming systems and a staple grain in the diet of sub-Saharan Africa and India. To facilitate breeding work in this crop, a genetic map consisting of single nucleotide polymorphism (SNP) markers was constructed using an F2 population of 93 progenies, from a wild × cultivated pearl millet cross. We used a modified genotyping-by-sequencing (GBS) protocol involving two restriction enzymes (PstI–MspI) and PCR amplification with primers including three selective bases to generate 3,321 SNPs. Of these, 2,809 high-quality SNPs exhibited a minor allele frequency ≥0.3. In total, 314 non-redundant haplotypes and 85 F2 individuals were used to construct a genetic map spanning a total distance of 640 cM. These SNPs were evenly distributed over seven linkage groups ranging considerably in size (62–123 cM). The average density for this map was 0.51 SNP/cM, and the average interval between SNP markers was 2.1 (±0.6) cM. Finally, to establish bridges between the linkage groups of this and previous maps, 19 SSR markers were examined for polymorphism between the parents of this population. We could only tentatively suggest a correspondence between four of our linkage groups and those of previous maps. Overall, GBS enabled us to quickly produce a genetic map with a density and uniformity of markers greater than previously published maps. The availability of such a map will be useful for the identification of genomic regions associated with Striga resistance and other important agronomic traits.
date: 2015
date_type: published
publication: Molecular Breeding
volume: 35
number: 5
publisher: Springer Netherlands
pagerange: 1-10
refereed: TRUE
issn: 1380-3743
official_url: http://dx.doi.org/10.1007/s11032-015-0212-x
related_url_url: http://scholar.google.co.in/scholar?as_q=Construction+of+a+genetic+map+for+pearl+millet%2C+Pennisetum+glaucum+%28L.%29+R.+Br.%2C+using+a+genotyping-by-sequencing+%28GBS%29+approach&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&a
related_url_type: pub
citation:   Moumouni, K H and Kountche, B A and Jean, M and Hash, C T and Vigouroux, Y and Haussmann, B I G and Belzile, F  (2015) Construction of a genetic map for pearl millet, Pennisetum glaucum (L.) R. Br., using a genotyping-by-sequencing (GBS) approach.  Molecular Breeding, 35 (5).  pp. 1-10.  ISSN 1380-3743     
document_url: http://oar.icrisat.org/9112/1/MolBreeding_35_5_2015.pdf