TY  - JOUR
AV  - restricted
UR  - http://dx.doi.org/10.1007/s11032-015-0212-x
IS  - 5
PB  - Springer Netherlands
TI  - Construction of a genetic map for pearl millet, Pennisetum glaucum (L.) R. Br., using a genotyping-by-sequencing (GBS) approach
A1  - Moumouni, K H
A1  - Kountche, B A
A1  - Jean, M
A1  - Hash, C T
A1  - Vigouroux, Y
A1  - Haussmann, B I G
A1  - Belzile, F
SN  - 1380-3743
VL  - 35
N2  - Pearl millet is the main component of traditional farming systems and a staple grain in the diet of sub-Saharan Africa and India. To facilitate breeding work in this crop, a genetic map consisting of single nucleotide polymorphism (SNP) markers was constructed using an F2 population of 93 progenies, from a wild × cultivated pearl millet cross. We used a modified genotyping-by-sequencing (GBS) protocol involving two restriction enzymes (PstI?MspI) and PCR amplification with primers including three selective bases to generate 3,321 SNPs. Of these, 2,809 high-quality SNPs exhibited a minor allele frequency ?0.3. In total, 314 non-redundant haplotypes and 85 F2 individuals were used to construct a genetic map spanning a total distance of 640 cM. These SNPs were evenly distributed over seven linkage groups ranging considerably in size (62?123 cM). The average density for this map was 0.51 SNP/cM, and the average interval between SNP markers was 2.1 (±0.6) cM. Finally, to establish bridges between the linkage groups of this and previous maps, 19 SSR markers were examined for polymorphism between the parents of this population. We could only tentatively suggest a correspondence between four of our linkage groups and those of previous maps. Overall, GBS enabled us to quickly produce a genetic map with a density and uniformity of markers greater than previously published maps. The availability of such a map will be useful for the identification of genomic regions associated with Striga resistance and other important agronomic traits.
SP  - 1
Y1  - 2015///
EP  - 10
ID  - icrisat9112
KW  - Pearl millet
KW  -  Genetic map
KW  -  SNP
KW  -  Genotyping-by-sequencing
JF  - Molecular Breeding
ER  -