"9112","12","archive","17",,,"disk0/00/00/91/12","2015-11-04 03:45:42","2015-11-04 03:45:42","2015-11-04 03:45:42","article",,,"show","Library-ICRISAT@CGIAR.ORG",,,"","","","","","","","","","",,,,"Moumouni","K H","","","","","","","Hash","C T","","","","",,,,,"","",,,,,"","","ICRISAT (Niamey)","Canada","Construction of a genetic map for pearl millet, Pennisetum glaucum (L.) R. Br., using a genotyping-by-sequencing (GBS) approach","pub","S1.5","D4","crp1.4","restricted",,,"Pearl millet, Genetic map, SNP, Genotyping-by-sequencing",,,"Pearl millet is the main component of traditional farming systems and a staple grain in the diet of sub-Saharan Africa and India. To facilitate breeding work in this crop, a genetic map consisting of single nucleotide polymorphism (SNP) markers was constructed using an F2 population of 93 progenies, from a wild × cultivated pearl millet cross. We used a modified genotyping-by-sequencing (GBS) protocol involving two restriction enzymes (PstI–MspI) and PCR amplification with primers including three selective bases to generate 3,321 SNPs. Of these, 2,809 high-quality SNPs exhibited a minor allele frequency ≥0.3. In total, 314 non-redundant haplotypes and 85 F2 individuals were used to construct a genetic map spanning a total distance of 640 cM. These SNPs were evenly distributed over seven linkage groups ranging considerably in size (62–123 cM). The average density for this map was 0.51 SNP/cM, and the average interval between SNP markers was 2.1 (±0.6) cM. Finally, to establish bridges between the linkage groups of this and previous maps, 19 SSR markers were examined for polymorphism between the parents of this population. We could only tentatively suggest a correspondence between four of our linkage groups and those of previous maps. Overall, GBS enabled us to quickly produce a genetic map with a density and uniformity of markers greater than previously published maps. The availability of such a map will be useful for the identification of genomic regions associated with Striga resistance and other important agronomic traits.","2015","published",,"Molecular Breeding","35","5","Springer Netherlands",,"1-10",,,,,,,,,,,"TRUE",,"1380-3743",,,,,,"","http://dx.doi.org/10.1007/s11032-015-0212-x","http://scholar.google.co.in/scholar?as_q=Construction+of+a+genetic+map+for+pearl+millet%2C+Pennisetum+glaucum+%28L.%29+R.+Br.%2C+using+a+genotyping-by-sequencing+%28GBS%29+approach&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&a","pub",,"","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
"9112",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Kountche","B A","","",,,,,,,,,,,,,,,,,,,,,,,"Institut de Recherche pour le Développement (Montpellier)","Niger",,,"s2.13",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"9112",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Jean","M","","",,,,,,,,,,,,,,,,,,,,,,,"Université Abdou Moumouni (Niamey)","France",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"9112",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Hash","C T","","",,,,,,,,,,,,,,,,,,,,,,,"University of Hohenheim (Stuttgart)","Germany",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"9112",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Vigouroux","Y","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"9112",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Haussmann","B I G","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"9112",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Belzile","F","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,