OAR@ICRISATEvaluation of Broad-Spectrum Streptomyces sp. for Plant Growth Promotion Traits in Chickpea (Cicer arietinum L.)SGopalakrishnanauthorVSrinivasauthorGAlekhyaauthorBPrakashauthorHKudapaauthorR KVarshneyauthorThe study evaluated three strains of Streptomyces (CAI-21, CAI-26 and MMA-32) for their plant growth promoting (PGP) traits in chickpea (Cicer arietinum L.) under field conditions in two consecutive post-rainy seasons, 2012–2013 and 2013–2014, to confirm colonizing ability in chickpea and to demonstrate gene expression profiles of indoleacetic acid, siderophore and β- 1,3-glucanase genes. The Streptomyces enhanced nodule number, nodule weight, root weight and shoot weight at 30 d after sowing (DAS) and plant height, pod number, pod weight, leaf area, leaf weight and stem weight at 60 DAS in both seasons over the uninoculated control. At crop maturity, the Streptomyces enhanced stover yield, grain yield and total dry matter in both seasons over the uninoculated control. In the rhizosphere, the biological and mineral nutrient activities, such as microbial biomass carbon, dehydrogenase activity, total nitrogen, available phosphorus and organic carbon were also found higher in plots inoculated with Streptomyces in both seasons over the uninoculated control plots. Scanning electron microscopy analysis revealed that the Streptomyces colonized the roots of chickpea. Quantitative real time PCR analysis on selected PGP genes of Streptomyces revealed up-regulation of β-1,3-glucanase gene in CAI-26, β-1,3-glucanase and siderophore genes (siderophore synthetase) in CAI-21 and β-1,3-glucanase, siderophore (siderophore synthetase) and indole-3-acetic acid (IAAH and IAAM) genes in MMA-32. The three Streptomyces demonstrated broad-spectrum PGP activity.Chickpea2015-09College of Agriculture, University of the PhilippinesArticle

For work being deposited by its own author: In self-archiving this collection of files and associated bibliographic metadata, I grant OAR@ICRISAT the right to store them and to make them permanently available publicly for free on-line. I declare that this material is my own intellectual property and I understand that OAR@ICRISAT does not assume any responsibility if there is any breach of copyright in distributing these files or metadata. (All authors are urged to prominently assert their copyright on the title page of their work.)

For work being deposited by someone other than its author: I hereby declare that the collection of files and associated bibliographic metadata that I am archiving at OAR@ICRISAT) is in the public domain. If this is not the case, I accept full responsibility for any breach of copyright that distributing these files or metadata may entail.

Clicking on the deposit button indicates your agreement to these terms.