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        <dc:title>Assessing the genetic diversity of 48 groundnut (Arachis hypogaea L.) genotypes in the Guinea savanna agro-ecology of Ghana, using microsatellite-based markers</dc:title>
        <dc:creator>Oteng-Frimpong, R</dc:creator>
        <dc:creator>Sriswathi, M</dc:creator>
        <dc:creator>Ntare, B R</dc:creator>
        <dc:creator>Dakora, F D</dc:creator>
        <dc:subject>Groundnut</dc:subject>
        <dc:description>Groundnut (Arachis hypogaea L.) is the most important grain legume in Ghana. However, its production&#13;
is constrained by a myriad of biotic and abiotic stresses which necessitate the development and use of&#13;
superior varieties for increased yield. Germplasm characterisation both at the phenotypic and molecular&#13;
level is important in all plant breeding programs. The aim of this study was to characterise selected&#13;
advanced breeding groundnut lines with different phenotypic attributes at the molecular level using&#13;
simple sequence repeats (SSR) markers in Ghana. A total of 53 SSR markers were screened and 25 were&#13;
found to be polymorphic with an average polymorphic information content (PIC) value of 0.57. Of the 48&#13;
groundnut genotypes studied, 67% showed very close relationship (~100% similarity) with one or more&#13;
genotypes among themselves. In fact, there were 14 instances where two to three genotypes within the&#13;
same sub-cluster exhibited 100% similarity even though they displayed different phenotypic attributes.&#13;
The remaining 33% of the groundnut genotypes were distant from each other and could therefore serve&#13;
as effective parental material for future work. In this study, the SSR-based markers were found to be&#13;
quite discriminatory in discerning variations between and among groundnut lines even where the level&#13;
of variation was low. Microsatellite-based markers therefore represent a useful tool for dissecting&#13;
genetic variations in cultivated crops, especially groundnut</dc:description>
        <dc:publisher>Academic Journals</dc:publisher>
        <dc:date>2015</dc:date>
        <dc:type>Article</dc:type>
        <dc:type>PeerReviewed</dc:type>
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        <dc:language>en</dc:language>
        <dc:identifier>http://oar.icrisat.org/9016/1/AfricanJBiotech_14_32_2484-2493_2015.pdf</dc:identifier>
        <dc:identifier>  Oteng-Frimpong, R and Sriswathi, M and Ntare, B R and Dakora, F D  (2015) Assessing the genetic diversity of 48 groundnut (Arachis hypogaea L.) genotypes in the Guinea savanna agro-ecology of Ghana, using microsatellite-based markers.  African Journal of Biotechnology, 14 (32).  pp. 2484-2493.  ISSN 1684-5315     </dc:identifier>
        <dc:relation>http://dx.doi.org/10.5897/AJB2015.14770</dc:relation>
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