eprintid: 8924
rev_number: 13
eprint_status: archive
userid: 1305
dir: disk0/00/00/89/24
datestamp: 2015-08-11 05:51:16
lastmod: 2016-10-21 04:45:16
status_changed: 2015-08-11 05:51:16
type: article
metadata_visibility: show
creators_name: Sehgal, D
creators_name: Skot, L
creators_name: Singh, R
creators_name: Srivastava, R K
creators_name: Das, S P
creators_name: Taunk, J
creators_name: Sharma, P C
creators_name: Pal, R
creators_name: Raj, B
creators_name: Hash, C T
creators_name: Yadav, R S
icrisatcreators_name: Srivastava, R K
icrisatcreators_name: Raj, B
icrisatcreators_name: Hash, C T
affiliation: Institute of Biological, Environmental and Biological Sciences (IBERS), Aberystwyth University (Aberystwyth)
affiliation: Chaudhary Charan Singh Haryana Agricultural University (CCSHAU), Department of Molecular Biology and Biotechnology (Hisar)
affiliation: ICRISAT(Patancheru)
affiliation: ICAR Research Complex for NEH Region, Tripura Centre (Lembucherra)
affiliation: Central Soil Salinity Research Institute (CSSRI) (Karnal)
affiliation: National Research Centre for Orchids, Darjeeling Campus (Darjeeling)
affiliation: ICRISAT(Niamey)
country: United Kingdom
country: India
country: Niger
title: Exploring Potential of Pearl Millet Germplasm Association Panel for Association Mapping of Drought Tolerance Traits
ispublished: pub
subjects: S1.5.1
divisions: D4
crps: crp1.4
full_text_status: public
keywords: Pearl Millet; Germplasm; Panicles; Africa, Drought; Leaves; India; Serine; Variant genotypes; Chlorophyll; Drought tolerance
note: The authors wish to acknowledge Biotechnology and Biological Sciences Research Council (BBSRC) and Department for International Development (DFID) for funding the work via grant. The authors wish to thank all support staff who contributed to the development of the PMiGAP, as well as to seed multiplication of the PMiGAP testcrosses and phenotyping of these testcrosses. The authors are also thankful to Mrs. Kirsten Skot for genotyping PMiGAP with SSRs and InDel markers at in-house facility at IBERS and P. Maheshwar Rao for phenotyping PMiGAP for leaf rolling and senescence.
abstract: A pearl millet inbred germplasm association panel (PMiGAP) comprising 250 inbred lines, representative of cultivated germplasm from Africa and Asia, elite improved open-pollinated cultivars, hybrid parental inbreds and inbred mapping population parents, was recently established. This study presents the first report of genetic diversity in PMiGAP and its exploitation for association mapping of drought tolerance traits. For diversity and genetic structure analysis, PMiGAP was genotyped with 37 SSR and CISP markers representing all seven linkage groups. For association analysis, it was phenotyped for yield and yield components and morpho-physiological traits under both well-watered and drought conditions, and genotyped with SNPs and InDels from seventeen genes underlying a major validated drought tolerance (DT) QTL. The average gene diversity in PMiGAP was 0.54. The STRUCTURE analysis revealed six subpopulations within PMiGAP. Significant associations were obtained for 22 SNPs and 3 InDels from 13 genes under different treatments. Seven SNPs associations from 5 genes were common under irrigated and one of the drought stress treatments. Most significantly, an important SNP in putative acetyl CoA carboxylase gene showed constitutive association with grain yield, grain harvest index and panicle yield under all treatments. An InDel in putative chlorophyll a/b binding protein gene was significantly associated with both stay-green and grain yield traits under drought stress. This can be used as a functional marker for selecting high yielding genotypes with ‘stay green’ phenotype under drought stress. The present study identified useful marker-trait associations of important agronomics traits under irrigated and drought stress conditions with genes underlying a major validated DT-QTL in pearl millet. Results suggest that PMiGAP is a useful panel for association mapping. Expression patterns of genes also shed light on some physiological mechanisms underlying pearl millet drought tolerance.
date: 2015-05
date_type: published
publication: PLoS ONE
publisher: Public Library of Science
pagerange: 1-28
id_number: 10.1371/journal.pone.0122165
refereed: TRUE
issn: 1932-6203
official_url: http://dx.doi.org/10.1371/journal.pone.0122165
related_url_url: https://scholar.google.co.in/scholar?hl=en&q=Exploring+Potential+of+Pearl+Millet+Germplasm+Association+Panel+for+Association+Mapping+of+Drought+Tolerance+Traits&btnG=
related_url_type: pub
funders: BBSRC and DFID
citation:   Sehgal, D and Skot, L and Singh, R and Srivastava, R K and Das, S P and Taunk, J and Sharma, P C and Pal, R and Raj, B and Hash, C T and Yadav, R S  (2015) Exploring Potential of Pearl Millet Germplasm Association Panel for Association Mapping of Drought Tolerance Traits.  PLoS ONE.  pp. 1-28.  ISSN 1932-6203     
document_url: http://oar.icrisat.org/8924/1/journal.pone.0122165.pdf