eprintid: 8892
rev_number: 14
eprint_status: archive
userid: 1305
dir: disk0/00/00/88/92
datestamp: 2015-07-22 06:18:39
lastmod: 2016-10-21 06:21:50
status_changed: 2015-07-22 06:18:39
type: article
metadata_visibility: show
creators_name: Bajaj, D
creators_name: Upadhyaya, H D
creators_name: Khan, Y
creators_name: Das, S
creators_name: Badoni, S
creators_name: Shree, T
creators_name: Kumar, V
creators_name: Tripathi, S
creators_name: Gowda, C L L
creators_name: Singh, S
creators_name: Sharma, S
creators_name: Tyagi, A K
creators_name: Chattopdhyay, D
creators_name: Parida, S K
icrisatcreators_name: Upadhyaya, H D
icrisatcreators_name: Gowda, C L L
icrisatcreators_name: Singh, S
icrisatcreators_name: Sharma, S
affiliation: National Institute of Plant Genome Research (NIPGR) (New Delhi)
affiliation: ICRISAT (Patancheru)
affiliation: National Research Centre on Plant Biotechnology (NRCPB) (New Delhi)
affiliation: Division of Genetics, Indian Agricultural Research Institute (IARI) (New Delhi)
country: India
title: A combinatorial approach of comprehensive QTL-based comparative genome mapping and transcript profiling identified a seed weight-regulating candidate gene in chickpea
ispublished: pub
subjects: s1.1
divisions: D3
crps: crp1.5
full_text_status: public
keywords: Genome Mapping; Chickpea; QTL mapping; Genotyping
note: The authors gratefully acknowledge the financial support by the Department of Biotechnology (DBT), Government of India, through their research grant (102/IFD/SAN/2161/2013-14) for this research work. SD acknowledge the DBT for Junior Research Fellowship award. We thank the DNA Sequencing Facility, NIPGR for automated fragment analysis and sequencing.
abstract: High experimental validation/genotyping success rate (94–96%) and intra-specific polymorphic potential (82–96%) of 1536 SNP and 472 SSR markers showing in silico polymorphism between desi ICC 4958 and kabuli ICC 12968 chickpea was obtained in a 190 mapping population (ICC 4958 × ICC 12968) and 92 diverse desi and kabuli genotypes. A high-density 2001 marker-based intra-specific genetic linkage map comprising of eight LGs constructed is comparatively much saturated (mean map-density: 0.94 cM) in contrast to existing intra-specific genetic maps in chickpea. Fifteen robust QTLs (PVE: 8.8–25.8% with LOD: 7.0–13.8) associated with pod and seed number/plant (PN and SN) and 100 seed weight (SW) were identified and mapped on 10 major genomic regions of eight LGs. One of 126.8 kb major genomic region harbouring a strong SW-associated robust QTL (Caq'SW1.1: 169.1–171.3 cM) has been delineated by integrating high-resolution QTL mapping with comprehensive marker-based comparative genome mapping and differential expression profiling. This identified one potential regulatory SNP (G/A) in the cis-acting element of candidate ERF (ethylene responsive factor) TF (transcription factor) gene governing seed weight in chickpea. The functionally relevant molecular tags identified have potential to be utilized for marker-assisted genetic improvement of chickpea.
date: 2015-03
date_type: published
publication: Scientific Reports
volume: 5
number: 9264
publisher: Nature Publishing Group
pagerange: 1-14
id_number: 10.1038/srep09264
refereed: TRUE
issn: 2045-2322
official_url: http://dx.doi.org/10.1038/srep09264
related_url_url: https://scholar.google.co.in/scholar?hl=en&q=A+combinatorial+approach+of+comprehensive+QTL-based+comparative+genome+mapping+and+transcript+profiling+identified+a+seed+weight-regulating+candidate+gene+in+chickpea&btnG=
related_url_type: pub
citation:   Bajaj, D and Upadhyaya, H D and Khan, Y and Das, S and Badoni, S and Shree, T and Kumar, V and Tripathi, S and Gowda, C L L and Singh, S and Sharma, S and Tyagi, A K and Chattopdhyay, D and Parida, S K  (2015) A combinatorial approach of comprehensive QTL-based comparative genome mapping and transcript profiling identified a seed weight-regulating candidate gene in chickpea.  Scientific Reports, 5 (9264).  pp. 1-14.  ISSN 2045-2322     
document_url: http://oar.icrisat.org/8892/1/srep09264.pdf