TY  - JOUR
AV  - public
UR  - http://dx.doi.org/10.1038/srep09264
IS  - 9264
PB  - Nature Publishing Group
TI  - A combinatorial approach of comprehensive QTL-based comparative genome mapping and transcript profiling identified a seed weight-regulating candidate gene in chickpea
A1  - Bajaj, D
A1  - Upadhyaya, H D
A1  - Khan, Y
A1  - Das, S
A1  - Badoni, S
A1  - Shree, T
A1  - Kumar, V
A1  - Tripathi, S
A1  - Gowda, C L L
A1  - Singh, S
A1  - Sharma, S
A1  - Tyagi, A K
A1  - Chattopdhyay, D
A1  - Parida, S K
N1  - The authors gratefully acknowledge the financial support by the Department of Biotechnology (DBT), Government of India, through their research grant (102/IFD/SAN/2161/2013-14) for this research work. SD acknowledge the DBT for Junior Research Fellowship award. We thank the DNA Sequencing Facility, NIPGR for automated fragment analysis and sequencing.
SN  - 2045-2322
VL  - 5
N2  - High experimental validation/genotyping success rate (94?96%) and intra-specific polymorphic potential (82?96%) of 1536 SNP and 472 SSR markers showing in silico polymorphism between desi ICC 4958 and kabuli ICC 12968 chickpea was obtained in a 190 mapping population (ICC 4958 × ICC 12968) and 92 diverse desi and kabuli genotypes. A high-density 2001 marker-based intra-specific genetic linkage map comprising of eight LGs constructed is comparatively much saturated (mean map-density: 0.94?cM) in contrast to existing intra-specific genetic maps in chickpea. Fifteen robust QTLs (PVE: 8.8?25.8% with LOD: 7.0?13.8) associated with pod and seed number/plant (PN and SN) and 100 seed weight (SW) were identified and mapped on 10 major genomic regions of eight LGs. One of 126.8?kb major genomic region harbouring a strong SW-associated robust QTL (Caq'SW1.1: 169.1?171.3?cM) has been delineated by integrating high-resolution QTL mapping with comprehensive marker-based comparative genome mapping and differential expression profiling. This identified one potential regulatory SNP (G/A) in the cis-acting element of candidate ERF (ethylene responsive factor) TF (transcription factor) gene governing seed weight in chickpea. The functionally relevant molecular tags identified have potential to be utilized for marker-assisted genetic improvement of chickpea.
SP  - 1
Y1  - 2015/03//
EP  - 14
ID  - icrisat8892
KW  - Genome Mapping; Chickpea; QTL mapping; Genotyping
JF  - Scientific Reports
ER  -