relation: http://oar.icrisat.org/8892/
title: A combinatorial approach of comprehensive QTL-based comparative genome mapping and transcript profiling identified a seed weight-regulating candidate gene in chickpea
creator: Bajaj, D
creator: Upadhyaya, H D
creator: Khan, Y
creator: Das, S
creator: Badoni, S
creator: Shree, T
creator: Kumar, V
creator: Tripathi, S
creator: Gowda, C L L
creator: Singh, S
creator: Sharma, S
creator: Tyagi, A K
creator: Chattopdhyay, D
creator: Parida, S K
subject: Chickpea
description: High experimental validation/genotyping success rate (94–96%) and intra-specific polymorphic potential (82–96%) of 1536 SNP and 472 SSR markers showing in silico polymorphism between desi ICC 4958 and kabuli ICC 12968 chickpea was obtained in a 190 mapping population (ICC 4958 × ICC 12968) and 92 diverse desi and kabuli genotypes. A high-density 2001 marker-based intra-specific genetic linkage map comprising of eight LGs constructed is comparatively much saturated (mean map-density: 0.94 cM) in contrast to existing intra-specific genetic maps in chickpea. Fifteen robust QTLs (PVE: 8.8–25.8% with LOD: 7.0–13.8) associated with pod and seed number/plant (PN and SN) and 100 seed weight (SW) were identified and mapped on 10 major genomic regions of eight LGs. One of 126.8 kb major genomic region harbouring a strong SW-associated robust QTL (Caq'SW1.1: 169.1–171.3 cM) has been delineated by integrating high-resolution QTL mapping with comprehensive marker-based comparative genome mapping and differential expression profiling. This identified one potential regulatory SNP (G/A) in the cis-acting element of candidate ERF (ethylene responsive factor) TF (transcription factor) gene governing seed weight in chickpea. The functionally relevant molecular tags identified have potential to be utilized for marker-assisted genetic improvement of chickpea.
publisher: Nature Publishing Group
date: 2015-03
type: Article
type: PeerReviewed
format: application/pdf
language: en
identifier: http://oar.icrisat.org/8892/1/srep09264.pdf
identifier:   Bajaj, D and Upadhyaya, H D and Khan, Y and Das, S and Badoni, S and Shree, T and Kumar, V and Tripathi, S and Gowda, C L L and Singh, S and Sharma, S and Tyagi, A K and Chattopdhyay, D and Parida, S K  (2015) A combinatorial approach of comprehensive QTL-based comparative genome mapping and transcript profiling identified a seed weight-regulating candidate gene in chickpea.  Scientific Reports, 5 (9264).  pp. 1-14.  ISSN 2045-2322     
relation: http://dx.doi.org/10.1038/srep09264
relation: 10.1038/srep09264