"8892","14","archive","1305",,,"disk0/00/00/88/92","2015-07-22 06:18:39","2016-10-21 06:21:50","2015-07-22 06:18:39","article",,,"show",,,,"","","","","","","","","","",,,,"Bajaj","D","","","","","","","Upadhyaya","H D","","","","",,,,,"","",,,,,"","","National Institute of Plant Genome Research (NIPGR) (New Delhi)","India","A combinatorial approach of comprehensive QTL-based comparative genome mapping and transcript profiling identified a seed weight-regulating candidate gene in chickpea","pub","s1.1","D3","crp1.5","public",,,"Genome Mapping; Chickpea; QTL mapping; Genotyping",,"The authors gratefully acknowledge the financial support by the Department of Biotechnology (DBT), Government of India, through their research grant (102/IFD/SAN/2161/2013-14) for this research work. SD acknowledge the DBT for Junior Research Fellowship award. We thank the DNA Sequencing Facility, NIPGR for automated fragment analysis and sequencing.","High experimental validation/genotyping success rate (94–96%) and intra-specific polymorphic potential (82–96%) of 1536 SNP and 472 SSR markers showing in silico polymorphism between desi ICC 4958 and kabuli ICC 12968 chickpea was obtained in a 190 mapping population (ICC 4958 × ICC 12968) and 92 diverse desi and kabuli genotypes. A high-density 2001 marker-based intra-specific genetic linkage map comprising of eight LGs constructed is comparatively much saturated (mean map-density: 0.94 cM) in contrast to existing intra-specific genetic maps in chickpea. Fifteen robust QTLs (PVE: 8.8–25.8% with LOD: 7.0–13.8) associated with pod and seed number/plant (PN and SN) and 100 seed weight (SW) were identified and mapped on 10 major genomic regions of eight LGs. One of 126.8 kb major genomic region harbouring a strong SW-associated robust QTL (Caq'SW1.1: 169.1–171.3 cM) has been delineated by integrating high-resolution QTL mapping with comprehensive marker-based comparative genome mapping and differential expression profiling. This identified one potential regulatory SNP (G/A) in the cis-acting element of candidate ERF (ethylene responsive factor) TF (transcription factor) gene governing seed weight in chickpea. The functionally relevant molecular tags identified have potential to be utilized for marker-assisted genetic improvement of chickpea.","2015-03","published",,"Scientific Reports","5","9264","Nature Publishing Group",,"1-14",,,,,,"10.1038/srep09264",,,,,"TRUE",,"2045-2322",,,,,,"","http://dx.doi.org/10.1038/srep09264","https://scholar.google.co.in/scholar?hl=en&q=A+combinatorial+approach+of+comprehensive+QTL-based+comparative+genome+mapping+and+transcript+profiling+identified+a+seed+weight-regulating+candidate+gene+in+chickpea&btnG=","pub",,"","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
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"8892",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Khan","Y","","",,,,,"Singh","S","","",,,,,,,,,,,,,,,"National Research Centre on Plant Biotechnology (NRCPB) (New Delhi)",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"8892",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Das","S","","",,,,,"Sharma","S","","",,,,,,,,,,,,,,,"Division of Genetics, Indian Agricultural Research Institute (IARI) (New Delhi)",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
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"8892",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Kumar","V","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
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"8892",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Gowda","C L L","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"8892",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Singh","S","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"8892",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Sharma","S","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"8892",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Tyagi","A K","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
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"8892",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Parida","S K","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,