@article{icrisat8892, month = {March}, year = {2015}, title = {A combinatorial approach of comprehensive QTL-based comparative genome mapping and transcript profiling identified a seed weight-regulating candidate gene in chickpea}, note = {The authors gratefully acknowledge the financial support by the Department of Biotechnology (DBT), Government of India, through their research grant (102/IFD/SAN/2161/2013-14) for this research work. SD acknowledge the DBT for Junior Research Fellowship award. We thank the DNA Sequencing Facility, NIPGR for automated fragment analysis and sequencing.}, volume = {5}, number = {9264}, pages = {1--14}, author = {D Bajaj and H D Upadhyaya and Y Khan and S Das and S Badoni and T Shree and V Kumar and S Tripathi and C L L Gowda and S Singh and S Sharma and A K Tyagi and D Chattopdhyay and S K Parida}, publisher = {Nature Publishing Group}, journal = {Scientific Reports}, url = {http://oar.icrisat.org/8892/}, abstract = {High experimental validation/genotyping success rate (94?96\%) and intra-specific polymorphic potential (82?96\%) of 1536 SNP and 472 SSR markers showing in silico polymorphism between desi ICC 4958 and kabuli ICC 12968 chickpea was obtained in a 190 mapping population (ICC 4958 {$\times$} ICC 12968) and 92 diverse desi and kabuli genotypes. A high-density 2001 marker-based intra-specific genetic linkage map comprising of eight LGs constructed is comparatively much saturated (mean map-density: 0.94 cM) in contrast to existing intra-specific genetic maps in chickpea. Fifteen robust QTLs (PVE: 8.8?25.8\% with LOD: 7.0?13.8) associated with pod and seed number/plant (PN and SN) and 100 seed weight (SW) were identified and mapped on 10 major genomic regions of eight LGs. One of 126.8 kb major genomic region harbouring a strong SW-associated robust QTL (Caq'SW1.1: 169.1?171.3 cM) has been delineated by integrating high-resolution QTL mapping with comprehensive marker-based comparative genome mapping and differential expression profiling. This identified one potential regulatory SNP (G/A) in the cis-acting element of candidate ERF (ethylene responsive factor) TF (transcription factor) gene governing seed weight in chickpea. The functionally relevant molecular tags identified have potential to be utilized for marker-assisted genetic improvement of chickpea.}, keywords = {Genome Mapping; Chickpea; QTL mapping; Genotyping} }