eprintid: 8846
rev_number: 13
eprint_status: archive
userid: 1305
dir: disk0/00/00/88/46
datestamp: 2015-07-10 04:09:18
lastmod: 2018-12-06 02:53:56
status_changed: 2015-07-10 04:09:18
type: article
metadata_visibility: show
creators_name: Khera, P
creators_name: Saxena, R K
creators_name: Sameer Kumar, C V
creators_name: Saxena, K B
creators_name: Varshney, R K
icrisatcreators_name: Khera, P
icrisatcreators_name: Saxena, R K
icrisatcreators_name: Sameer Kumar, C V
icrisatcreators_name: Saxena, K B
icrisatcreators_name: Varshney, R K
affiliation: ICRISAT (Patancheru)
country: India
title: Mitochondrial SSRs and their utility in distinguishing wild species, CMS lines and maintainer lines in pigeonpea (Cajanus cajan L.)
ispublished: pub
subjects: s1.2
divisions: D3
crps: crp1.5
full_text_status: restricted
keywords: Genetic diversity; Hybrid; Mitochondria; Molecular marker; Cytoplasmic male sterile; CMS
abstract: Analysis of the pigeonpea mitochondrial genome sequence identified 25 SSRs. Mononucleotide SSR motifs were the most abundant repeats followed by dinucleotide and trinucleotide repeats. Primer pairs could be designed for 24 SSRs, 23 of which were polymorphic amongst the 22 genotypes consisting of cytoplasmic male sterile (CMS or A) line, maintainer or B line and wild Cajanus species representing six different CMS systemsviz.,A1,A2,A 4,A5,A6 andA8. These markers amplified a total of 107 alleles ranging from 2 to 10 with an average of 4.65 alleles per locus. The polymorphic information content for these markers ranged from 0.09 to 0.84 with an average of 0.52 per marker. Hence, the present study adds a novel set of 24 mitochondrial SSR markers to the markers repository in pigeonpea, which would be useful to distinguish the genotypes based on mitochondrial genome types in evolutionary and phylogenetic studies.
date: 2015-07-07
publication: Euphytica
publisher: Springer Netherlands
pagerange: 1-10
refereed: TRUE
issn: 1573-5060
official_url: http://dx.doi.org/10.1007/s10681-015-1504-2
related_url_url: https://scholar.google.co.in/scholar?as_q=Mitochondrial+SSRs+and+their+utility+in+distinguishing+wild+species+CMS+lines+and+maintainer+lines+in+pigeonpea+Cajanus+cajan+L&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&btnG
related_url_type: pub
citation:   Khera, P and Saxena, R K and Sameer Kumar, C V and Saxena, K B and Varshney, R K  (2015) Mitochondrial SSRs and their utility in distinguishing wild species, CMS lines and maintainer lines in pigeonpea (Cajanus cajan L.).  Euphytica.  pp. 1-10.  ISSN 1573-5060     
document_url: http://oar.icrisat.org/8846/1/2015%20Euphytica%20Khera%20er%20al.%20MitSSR.pdf