eprintid: 8804
rev_number: 11
eprint_status: archive
userid: 1305
dir: disk0/00/00/88/04
datestamp: 2015-06-22 06:21:22
lastmod: 2015-08-06 09:59:29
status_changed: 2015-06-22 06:21:22
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
creators_name: Barve, M P
creators_name: Haware, M P
creators_name: Sainani, M N
creators_name: Ranjekar, P K
creators_name: Gupta, V S
icrisatcreators_name: Haware, M P
affiliation: Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory (Pune)
affiliation: ICRISAT (Patancheru)
country: India
title: Potential of microsatellites to distinguish four races of Fusarium oxysporum f. sp. ciceri prevalent in India
ispublished: pub
subjects: s1.1
divisions: D3
crps: crp1.5
full_text_status: restricted
keywords: Fusarium oxysporum; DNA fingerprinting; chickpea
abstract: Fusarium oxysporum f. sp. ciceri, the causal agent of chickpea wilt, is an important fungal pathogen in India. Thirteen oligonucleotide probes complementary to microsatellite loci, in combination with 11 restriction enzymes, were used to assess the potential of such markers to study genetic variability in four Indian races of the pathogen. Hybridisation patterns, which were dependent upon both the restriction enzyme and oligonucleotide probe used, revealed the presence of different repeat motifs in the F. oxysporum f. sp. ciceri genome. Among the restriction enzymes used, hexa-cutting enzymes were more informative than tetra- and penta-cutting enzymes, whereas tetranucleotide and trinucleotide repeats yielded better hybridisation patterns than dinucleotide repeats. Dependent upon the levels of polymorphism detected, we have identified (AGT)5, (ATC)5 and (GATA)4 as the best fingerprinting probes for the F. oxysporum f. sp. ciceri races. The distribution of microsatellite repeats in the genome revealed races 1 and 4 to be closely related at a similarity index value of 76.6%, as compared to race 2 at a similarity value of 67.3%; race 3 was very distinct at a similarity value of 26.7%. Our study demonstrates the potential of oligonucleotide probes for fingerprinting and studying variability in the F. oxysporum f. sp. ciceri races and represents a step towards the identification of potential race diagnostic markers.
date: 2001-01
publication: Theoretical and Applied Genetics
volume: 102
number: 1
publisher: Springer-Verlag
pagerange: 138-147
refereed: TRUE
issn: 1432-2242
official_url: http://dx.doi.org/10.1007/s001220051629
related_url_url: https://scholar.google.co.in/scholar?as_q=Potential+of+microsatellites+to+distinguish+four+races+of+Fusarium+oxysporum+f+sp+ciceri+prevalent+in+India&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&btnG=&hl=en&as_sdt=0%2C5
related_url_type: pub
citation:   Barve, M P and Haware, M P and Sainani, M N and Ranjekar, P K and Gupta, V S  (2001) Potential of microsatellites to distinguish four races of Fusarium oxysporum f. sp. ciceri prevalent in India.  Theoretical and Applied Genetics, 102 (1).  pp. 138-147.  ISSN 1432-2242     
document_url: http://oar.icrisat.org/8804/1/art%253A10.1007%252Fs001220051629.pdf