eprintid: 8725
rev_number: 10
eprint_status: archive
userid: 1305
dir: disk0/00/00/87/25
datestamp: 2015-05-15 04:11:57
lastmod: 2016-04-21 11:00:26
status_changed: 2015-05-15 04:11:57
type: article
metadata_visibility: show
creators_name: Nagaraju, M
creators_name: Reddy, P S
creators_name: Anil Kumar, S
creators_name: Srivastava, R K
creators_name: Kavi Kishor, P B
creators_name: Rao, D M
icrisatcreators_name: Reddy, P S
icrisatcreators_name: Srivastava, R K
affiliation: ICRISAT(Patancheru)
affiliation: Osmania University (Hyderabad)
country: India
title: Genome-wide Scanning and Characterization of Sorghum bicolor L. Heat Shock Transcription Factors
ispublished: pub
subjects: s1.4
subjects: s2.13
divisions: D4
crps: crp1.4
full_text_status: public
keywords: Heat shock transcription factors, Phylogenetic analysis, Cis-acting elements, Paralogs, Molecular chaperones, Abiotic stress
note: MN is thankful to the UGC, New Delhi, for providing
fellowship. PSR is thankful to the Department of Science
and Technology, Govt. of India, New Delhi, for research
funding through the INSPIRE Faculty Award Grant No.
IFA-11LSPA-06. PBK is thankful to the CSIR, New Delhi,
for providing Emeritus Fellowship. DMR is thankful to the
UGC, New Delhi, for financial support through UGCMRP.
abstract: A genome-wide scanning of Sorghum bicolor resulted in the identification of 25 SbHsf
genes. Phylogenetic analysis shows the ortholog genes that are clustered with only rice, representing a
common ancestor. Promoter analysis revealed the identification of different cis-acting elements that
are responsible for abiotic as well as biotic stresses. Hsf domains like DBD, NLS, NES, and AHA
have been analyzed for their sequence similarity and functional characterization. Tissue specific expression
patterns of Hsfs in different tissues like mature embryo, seedling, root, and panicle were studied
using real-time PCR. While Hsfs4 and 22 are highly expressed in panicle, 4 and 9 are expressed in
seedlings. Sorghum plants were exposed to different abiotic stress treatments but no expression of any Hsf was observed
when seedlings were treated with ABA. High level expression of Hsf1 was noticed during high temperature as well as
cold stresses, 4 and 6 during salt and 5, 6, 10, 13, 19, 23 and 25 during drought stress. This comprehensive analysis of
SbHsf genes will provide an insight on how these genes are regulated in different tissues and also under different abiotic
stresses and help to determine the functions of Hsfs during drought and temperature stress tolerance.
date: 2015
date_type: published
publication: Current Genomics
volume: 16
number: 4
publisher: Bentham Science
pagerange: 279-291
id_number: http://dx.doi.org/10.2174/1389202916666150313230812
refereed: TRUE
issn: 1875-5488
official_url: http://benthamscience.com/journal/abstracts.php?journalID=cg&articleID=129505
related_url_url: https://scholar.google.co.in/scholar?as_q=Genome+wide+Scanning+and+Characterization+of+Sorghum+bicolor+L+Heat+Shock+Transcription+Factors&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&btnG=&hl=en&as_sdt=0%2C5
related_url_type: pub
citation:   Nagaraju, M and Reddy, P S and Anil Kumar, S and Srivastava, R K and Kavi Kishor, P B and Rao, D M  (2015) Genome-wide Scanning and Characterization of Sorghum bicolor L. Heat Shock Transcription Factors.  Current Genomics, 16 (4).  pp. 279-291.  ISSN 1875-5488     
document_url: http://oar.icrisat.org/8725/1/CG-16-279.pdf