http://oar.icrisat.org/id/eprint/8694 2015-08-06T08:27:55Z info:eu-repo/semantics/descriptiveMetadata http://oar.icrisat.org/8694/ Deploying QTL-seq for rapid delineation of a potential candidate gene underlying major trait-associated QTL in chickpea Das, S Upadhyaya, H D Bajaj, D Kujur, A Badoni, S Laxmi, A Kumar, V Tripathi, S Laxmipathi Gowda, C L Sharma, S Singh, S Tyagi, A K Parida, S K Chickpea A rapid high-resolution genome-wide strategy for molecular mapping of major QTL(s)/gene(s) regulating important agronomic traits is vital for in-depth dissection of complex quantitative traits and genetic enhancement in chickpea. The present study for the first time employed a NGS-based whole-genomeQTL-seq strategy to identify one major genomic region harbouring a robust 100- seed weight QT Lusinganintra-specific 221 chickpea mapping population (desicv.ICC7184×desicv.ICC 15061). The QTL-seq-derived major SW QTL (CaqSW1.1) was further validated by single-nucleotide polymorphism (SNP) and simple sequence repeat (SSR) marker-based traditional QTL mapping (47.6% R2 at higher LOD >19). This reflects the reliability and efficacy of QTL-seq as a strategy for rapid genome-wide scanning and fine mapping of major trait regulatory QTLs in chickpea. The use of QTL-seq and classical QTL mapping in combination narrowed down the 1.37 Mb (comprising 177genes) major SWQTL (CaqSW1.1) regionintoa 35 kb genomic intervalondesi chickpea chromosome 1 containing six genes. One coding SNP (G/A)-carrying constitutive photomorphogenic 9 (COP9) signalo some complex subunit (CSN8) gene of the see xhibited seed-specific expression, including pronounced differential up-/down-regulation in low and high seed weight mapping parents and homo zygous individuals duringseed development.The coding SNP mined in this potential seed weight- governing candidate CSN8 genewas found to be present exclusively in all cultivated species/ genotypes, but notin any wild species/genotypes of primary, secondary and tertiary gene pools.This indicates the effect of strong artificial and/or natural selection pressure on target SW locus during chickpea domestication. The proposed QTL-seq-driven integrated genome-wide strategy has potential to delineate major candidate gene(s) harbouring a robust trait regulatory QTL rapidly with optimal use of resources. This will further assist us to extrapolate the molecular mechanism underlying complex quantitative traits at a genome-wide scale leading to fast-paced marker-assisted genetic improvement in diverse crop plants, including chickpea. Oxford University Press 2015-04 Article PeerReviewed application/pdf en http://oar.icrisat.org/8694/1/DNA%20Res-2015-Das-dnares-dsv004.pdf Das, S and Upadhyaya, H D and Bajaj, D and Kujur, A and Badoni, S and Laxmi, A and Kumar, V and Tripathi, S and Laxmipathi Gowda, C L and Sharma, S and Singh, S and Tyagi, A K and Parida, S K (2015) Deploying QTL-seq for rapid delineation of a potential candidate gene underlying major trait-associated QTL in chickpea. DNA Research, 22 (2). pp. 1-11. ISSN 1340-2838 http://dx.doi.org/10.1093/dnares/dsv004 http://dx.doi.org/doi: 10.1093/dnares/dsv004 info:eu-repo/semantics/objectFile info:eu-repo/semantics/humanStartPage