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dc:title "HTML Summary of #8664 \n\nIdentification of groundnut (Arachis hypogaea) SSR markers suitable for multiple resistance traits QTL mapping in African germplasm\n\n";
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bibo:abstract "Background: This study aimed to identify and select informative Simple Sequence Repeat (SSR)markers that may\r\nbe linked to resistance to important groundnut diseases such as Early Leaf Spot, Groundnut Rosette Disease, rust\r\nand aflatoxin contamination. To this end, 799 markers were screened across 16 farmer preferred and other\r\ncultivated African groundnut varieties that are routinely used in groundnut improvement, some with known\r\nresistance traits.\r\nResults: The SSR markers amplified 817 loci and were graded on a scale of 1 to 4 according to successful\r\namplification and ease of scoring of amplified alleles. Of these, 376 markers exhibited Polymorphic\r\nInformation Content (PIC) values ranging from 0.06 to 0.86, with 1476 alleles detected at an average of 3.7\r\nalleles per locus. The remaining 423 markers were either monomorphic or did not work well. The best\r\nperforming polymorphic markers were subsequently used to construct a dissimilarity matrix that indicated\r\nthe relatedness of the varieties in order to aid selection of appropriately diverse parents for groundnut\r\nimprovement. The closest related varieties were MGV5 and ICGV-SM 90704 and most distant were\r\nChalimbana and 47–10. The mean dissimilarity value was 0.51, ranging from 0.34 to 0.66.\r\nDiscussion: Of the 376 informative markers identified in this study, 139 (37%) have previously been mapped to\r\nthe Arachis genome and can now be employed in Quantitative Trait Loci (QTL) mapping and the additional\r\n237 markers identified can be used to improve the efficiency of introgression of resistance to multiple\r\nimportant biotic constraints into farmer-preferred varieties of Sub-Saharan Africa"^^xsd:string;
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