eprintid: 8659
rev_number: 25
eprint_status: archive
userid: 17
dir: disk0/00/00/86/59
datestamp: 2015-04-16 05:26:27
lastmod: 2018-02-05 01:15:52
status_changed: 2015-04-16 05:26:27
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
creators_name: Bayer, P E
creators_name: Ruperao, P
creators_name: Mason, A S
creators_name: Stiller, J
creators_name: Chan, C K K
creators_name: Hayashi, S
creators_name: Long, Y
creators_name: Meng, J
creators_name: Sutton, T
creators_name: Visendi, P
creators_name: Varshney, R K
creators_name: Batley, J
creators_name: Edwards, D
icrisatcreators_name: Ruperao, P
icrisatcreators_name: Varshney, R K
affiliation: School of Agriculture and Food Sciences, University of Queensland (Brisbane)
affiliation: Australian Centre for Plant Functional Genomics, School of Agriculture and Food Sciences, University of Queensland (Brisbane)
affiliation: University of Western Australia (Perth)
affiliation: ICRISAT (Patancheru)
affiliation: Centre for Integrative Legume Research, University of Queensland (Brisbane)
affiliation: Commonwealth Scientific and Industrial Research Organisation (Brisbane)
affiliation: Huazhong Agricultural University (Wuhan)
affiliation: South Australian Research and Development Institute (Adelaide)
affiliation: University of Adelaide (Glen Osmond)
country: Australia
country: India
country: China
title: High‑resolution skim genotyping by sequencing reveals the
distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus
ispublished: pub
subjects: s2.13
divisions: D3
crps: crp1.5
full_text_status: restricted
abstract: The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene-trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.
date: 2015
date_type: published
publication: Theoretical and Applied Genetics
volume: 128
number: 6
publisher: Springer-Verlag Berlin Heidelberg
pagerange: 1039-1047
refereed: TRUE
issn: 0040-1706
official_url: http://dx.doi.org/10.1007/s00122-015-2488-y
related_url_url: http://scholar.google.co.in/scholar?as_q=High%E2%80%91resolution+skim+genotyping+by+sequencing+reveals+the+distribution+of+crossovers+and+gene+conversions+in+Cicer+arietinum+and+Brassica+napus&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publicatio
related_url_type: pub
citation:   Bayer, P E and Ruperao, P and Mason, A S and Stiller, J and Chan, C K K and Hayashi, S and Long, Y and Meng, J and Sutton, T and Visendi, P and Varshney, R K and Batley, J and Edwards, D  (2015) High‑resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus.  Theoretical and Applied Genetics, 128 (6).  pp. 1039-1047.  ISSN 0040-1706     
document_url: http://oar.icrisat.org/8659/1/TAG_128_1039-1047_2015.pdf