%D 2015
%L icrisat8659
%I Springer-Verlag Berlin Heidelberg
%T High‑resolution skim genotyping by sequencing reveals the
distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus
%P 1039-1047
%V 128
%N 6
%A P E Bayer
%A P Ruperao
%A A S Mason
%A J Stiller
%A C K K Chan
%A S Hayashi
%A Y Long
%A J Meng
%A T Sutton
%A P Visendi
%A R K Varshney
%A J Batley
%A D Edwards
%J Theoretical and Applied Genetics
%X The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene-trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.