http://oar.icrisat.org/id/eprint/8659 2018-02-05T01:15:52Z info:eu-repo/semantics/descriptiveMetadata http://oar.icrisat.org/8659/ High‑resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus Bayer, P E Ruperao, P Mason, A S Stiller, J Chan, C K K Hayashi, S Long, Y Meng, J Sutton, T Visendi, P Varshney, R K Batley, J Edwards, D Genetics and Genomics The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene-trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations. Springer-Verlag Berlin Heidelberg 2015 Article PeerReviewed application/pdf en http://oar.icrisat.org/8659/1/TAG_128_1039-1047_2015.pdf Bayer, P E and Ruperao, P and Mason, A S and Stiller, J and Chan, C K K and Hayashi, S and Long, Y and Meng, J and Sutton, T and Visendi, P and Varshney, R K and Batley, J and Edwards, D (2015) High‑resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus. Theoretical and Applied Genetics, 128 (6). pp. 1039-1047. ISSN 0040-1706 http://dx.doi.org/10.1007/s00122-015-2488-y info:eu-repo/semantics/objectFile info:eu-repo/semantics/humanStartPage