relation: http://oar.icrisat.org/8659/
title: High‑resolution skim genotyping by sequencing reveals the  distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus
creator: Bayer, P E
creator: Ruperao, P
creator: Mason, A S
creator: Stiller, J
creator: Chan, C K K
creator: Hayashi, S
creator: Long, Y
creator: Meng, J
creator: Sutton, T
creator: Visendi, P
creator: Varshney, R K
creator: Batley, J
creator: Edwards, D
subject: Genetics and Genomics
description: The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene-trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.
publisher: Springer-Verlag Berlin Heidelberg
date: 2015
type: Article
type: PeerReviewed
format: application/pdf
language: en
identifier: http://oar.icrisat.org/8659/1/TAG_128_1039-1047_2015.pdf
identifier:   Bayer, P E and Ruperao, P and Mason, A S and Stiller, J and Chan, C K K and Hayashi, S and Long, Y and Meng, J and Sutton, T and Visendi, P and Varshney, R K and Batley, J and Edwards, D  (2015) High‑resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus.  Theoretical and Applied Genetics, 128 (6).  pp. 1039-1047.  ISSN 0040-1706     
relation: http://dx.doi.org/10.1007/s00122-015-2488-y