@article{icrisat8659, year = {2015}, title = {High?resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus}, volume = {128}, number = {6}, pages = {1039--1047}, author = {P E Bayer and P Ruperao and A S Mason and J Stiller and C K K Chan and S Hayashi and Y Long and J Meng and T Sutton and P Visendi and R K Varshney and J Batley and D Edwards}, publisher = {Springer-Verlag Berlin Heidelberg}, journal = {Theoretical and Applied Genetics}, url = {http://oar.icrisat.org/8659/}, abstract = {The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene-trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.} }