eprintid: 8594
rev_number: 20
eprint_status: archive
userid: 1305
dir: disk0/00/00/85/94
datestamp: 2015-03-10 11:09:57
lastmod: 2017-09-07 10:04:40
status_changed: 2015-03-10 11:09:57
type: article
metadata_visibility: show
creators_name: De Villiers, S M
creators_name: Michael, V N
creators_name: Manyasa, E O
creators_name: Saiyiorri, A N
creators_name: Deshpande, S P
icrisatcreators_name: De Villiers, S M
icrisatcreators_name: Michael, V N
icrisatcreators_name: Manyasa, E O
icrisatcreators_name: Saiyiorri, A N
icrisatcreators_name: Deshpande, S P
affiliation: ICRISAT(Nairobi)
affiliation: ICRISAT(Nairobi)
affiliation: ICRISAT(Nairobi)
affiliation: ICRISAT(Nairobi)
affiliation: ICRISAT(Patancheru)
country: Kenya
country: India
title: Compilation of an informative microsatellite set for genetic characterisation of East African finger millet (Eleusine coracana)
ispublished: pub
subjects: S1.5.2
divisions: D4
crps: crp1.4
full_text_status: public
keywords: Diversity analysis; SSR markers; Genotyping ; East Africa, Finger millet
abstract: Genetic diversity of finger millet (Eleusine coracana), a nutritious neglected staple cereal in Africa
and South Asia is largely uncharacterized. This study analysed 82 published SSR markers for finger millet
across 10 diverse accessions to compile an informative set for genetic characterisation. Extensive optimization
compared single samples with bulked leaf or bulked DNA samples for capturing within accession genetic
diversity. The markers were evaluated to determine (1) how efficiently they amplified target loci during
high-throughput genotyping with a generic PCR protocol, (2) ease of scoring PCR products and (3)
polymorphism and ability to discern genetic diversity within the tested finger millet germplasm.
date: 2015-03
date_type: published
publication: Electronic Journal of Biotechnology
volume: 18
number: 2
publisher: Pontificia Universidad Católica de Valparaíso
pagerange: 77-82
id_number: 10.1016/j.ejbt.2014.12.001
refereed: TRUE
issn: 0717-3458
official_url: http://dx.doi.org/10.1016/j.ejbt.2014.12.001
related_url_url: https://scholar.google.co.in/scholar?as_q=Compilation+of+an+informative+microsatellite+set+for+genetic+characterisation+of+East+African+finger+millet+Eleusine+coracana&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&btnG=&
related_url_type: pub
funders: Open Access funded by Pontificia Universidad Católica de Valparaíso
citation:   De Villiers, S M and Michael, V N and Manyasa, E O and Saiyiorri, A N and Deshpande, S P  (2015) Compilation of an informative microsatellite set for genetic characterisation of East African finger millet (Eleusine coracana).  Electronic Journal of Biotechnology, 18 (2).  pp. 77-82.  ISSN 0717-3458     
document_url: http://oar.icrisat.org/8594/1/Compilation%20of%20an%20informative%20microsatellite.pdf