TY  - JOUR
AV  - public
UR  - http://dx.doi.org/10.1016/j.ejbt.2014.12.001
IS  - 2
PB  - Pontificia Universidad Católica de Valparaíso
TI  - Compilation of an informative microsatellite set for genetic characterisation of East African finger millet (Eleusine coracana)
A1  - De Villiers, S M
A1  - Michael, V N
A1  - Manyasa, E O
A1  - Saiyiorri, A N
A1  - Deshpande, S P
SN  - 0717-3458
VL  - 18
N2  - Genetic diversity of finger millet (Eleusine coracana), a nutritious neglected staple cereal in Africa
and South Asia is largely uncharacterized. This study analysed 82 published SSR markers for finger millet
across 10 diverse accessions to compile an informative set for genetic characterisation. Extensive optimization
compared single samples with bulked leaf or bulked DNA samples for capturing within accession genetic
diversity. The markers were evaluated to determine (1) how efficiently they amplified target loci during
high-throughput genotyping with a generic PCR protocol, (2) ease of scoring PCR products and (3)
polymorphism and ability to discern genetic diversity within the tested finger millet germplasm.
SP  - 77
Y1  - 2015/03//
EP  - 82
ID  - icrisat8594
KW  - Diversity analysis; SSR markers; Genotyping ; East Africa
KW  -  Finger millet
JF  - Electronic Journal of Biotechnology
ER  -