http://oar.icrisat.org/id/eprint/8594 2017-09-07T10:04:40Z info:eu-repo/semantics/descriptiveMetadata http://oar.icrisat.org/8594/ Compilation of an informative microsatellite set for genetic characterisation of East African finger millet (Eleusine coracana) De Villiers, S M Michael, V N Manyasa, E O Saiyiorri, A N Deshpande, S P Finger Millet Genetic diversity of finger millet (Eleusine coracana), a nutritious neglected staple cereal in Africa and South Asia is largely uncharacterized. This study analysed 82 published SSR markers for finger millet across 10 diverse accessions to compile an informative set for genetic characterisation. Extensive optimization compared single samples with bulked leaf or bulked DNA samples for capturing within accession genetic diversity. The markers were evaluated to determine (1) how efficiently they amplified target loci during high-throughput genotyping with a generic PCR protocol, (2) ease of scoring PCR products and (3) polymorphism and ability to discern genetic diversity within the tested finger millet germplasm. Pontificia Universidad Católica de Valparaíso 2015-03 Article PeerReviewed application/pdf en http://oar.icrisat.org/8594/1/Compilation%20of%20an%20informative%20microsatellite.pdf De Villiers, S M and Michael, V N and Manyasa, E O and Saiyiorri, A N and Deshpande, S P (2015) Compilation of an informative microsatellite set for genetic characterisation of East African finger millet (Eleusine coracana). Electronic Journal of Biotechnology, 18 (2). pp. 77-82. ISSN 0717-3458 http://dx.doi.org/10.1016/j.ejbt.2014.12.001 10.1016/j.ejbt.2014.12.001 info:eu-repo/semantics/objectFile info:eu-repo/semantics/humanStartPage