relation: http://oar.icrisat.org/8594/
title: Compilation of an informative microsatellite set for genetic characterisation of East African finger millet (Eleusine coracana)
creator: De Villiers, S M
creator: Michael, V N
creator: Manyasa, E O
creator: Saiyiorri, A N
creator: Deshpande, S P
subject: Finger Millet
description: Genetic diversity of finger millet (Eleusine coracana), a nutritious neglected staple cereal in Africa  and South Asia is largely uncharacterized. This study analysed 82 published SSR markers for finger millet  across 10 diverse accessions to compile an informative set for genetic characterisation. Extensive optimization  compared single samples with bulked leaf or bulked DNA samples for capturing within accession genetic  diversity. The markers were evaluated to determine (1) how efficiently they amplified target loci during  high-throughput genotyping with a generic PCR protocol, (2) ease of scoring PCR products and (3)  polymorphism and ability to discern genetic diversity within the tested finger millet germplasm.
publisher: Pontificia Universidad Católica de Valparaíso
date: 2015-03
type: Article
type: PeerReviewed
format: application/pdf
language: en
identifier: http://oar.icrisat.org/8594/1/Compilation%20of%20an%20informative%20microsatellite.pdf
identifier:   De Villiers, S M and Michael, V N and Manyasa, E O and Saiyiorri, A N and Deshpande, S P  (2015) Compilation of an informative microsatellite set for genetic characterisation of East African finger millet (Eleusine coracana).  Electronic Journal of Biotechnology, 18 (2).  pp. 77-82.  ISSN 0717-3458     
relation: http://dx.doi.org/10.1016/j.ejbt.2014.12.001
relation: 10.1016/j.ejbt.2014.12.001