eprintid: 8570 rev_number: 25 eprint_status: archive userid: 1305 dir: disk0/00/00/85/70 datestamp: 2015-02-24 08:37:13 lastmod: 2017-11-21 08:25:01 status_changed: 2015-02-24 08:37:47 type: article metadata_visibility: show contact_email: Library-ICRISAT@CGIAR.ORG creators_name: Sudini, H creators_name: Srilakshmi, P creators_name: Vijay Krishna Kumar, K creators_name: Njoroge, S M C creators_name: Osiru, M creators_name: Anitha, S creators_name: Waliyar, F icrisatcreators_name: Sudini, H icrisatcreators_name: Srilakshmi, P icrisatcreators_name: Vijay Krishna Kumar, K icrisatcreators_name: Njoroge, S M C icrisatcreators_name: Osiru, M icrisatcreators_name: Anitha, S icrisatcreators_name: Waliyar, F affiliation: ICRISAT(Patancheru) affiliation: ICRISAT(Lilongwe) affiliation: ICRISAT(Bamako) country: India country: Malawi country: Mali title: Detection of aflatoxigenic Aspergillus strains by cultural and molecular methods: A critical review ispublished: pub subjects: s2.10 subjects: s2.15 subjects: s2.17 subjects: s2.4 divisions: D3 crps: crp1.5 full_text_status: public keywords: Toxigenic Aspergillus, atoxigenic Aspergillus, cultural methods, PCR based methods abstract: Aflatoxin contamination of food and feed commodities, caused by Aspergillus section Flavi group of fungi, is a serious problem worldwide. Exposure through consumption of contaminated food and feed has deleterious effects on human and animal health. Therefore, aflatoxin contaminated products are a barrier to international trade of agricultural commodities. Not all fungi from Aspergillus section Flavi produce aflatoxins. Hence it is important to differentiate Aspergillus spp. into toxigenic and atoxigenic species to better understand their population structure in a specific environment. A range of methods are available today, including cultural, analytical and molecular methods, to identify the toxin producing ability of isolates from section Flavi. A comprehensive review of these methods would be of great use for researchers in developing nations where fully equipped aflatoxin detection laboratories are lacking. In this paper we critically reviewed the cultural and molecular methods of detecting aflatoxigenic Aspergillus species and their precision. date: 2015-02-25 date_type: published publication: African Journal of Microbiology Research volume: 9 number: 8 publisher: Academic Journals pagerange: 484-491 id_number: 10.5897/AJMR2014.7309 refereed: TRUE issn: 1996-0808 official_url: http://dx.doi.org/10.5897/AJMR2014.7309 related_url_url: https://scholar.google.co.in/scholar?as_q=%22Detection+of+aflatoxigenic+Aspergillus+strains+by+cultural+and+molecular+methods%3A+A+critical+review&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&btnG=&hl=en&as_sdt=0%2C5 related_url_type: pub citation: Sudini, H and Srilakshmi, P and Vijay Krishna Kumar, K and Njoroge, S M C and Osiru, M and Anitha, S and Waliyar, F (2015) Detection of aflatoxigenic Aspergillus strains by cultural and molecular methods: A critical review. African Journal of Microbiology Research, 9 (8). pp. 484-491. ISSN 1996-0808 document_url: http://oar.icrisat.org/8570/1/AJMR_9_8_484-491_2015.pdf