OAR@ICRISATTechniques for diagnosis of Pseudomonas solanacearum and for resistance screening against groundnut bacterial wilt: training manualV KMehanauthorDMcDonaldauthorIn this manual, we present a comprehensive range of methods for isolation, identification, and detection of the groundnut bacterial wilt pathogen, Pseudomonas solanacearum. Emphasis has been placed on the use ofenzymelinked immunosorbent assay (ELISA) to detect the bacterium in plant tissues, seed, and soil. Some of the techniques described have been in use for some time without undergoing any major improvements, while others are of relatively recent origin. The principles under lying the major improvements, while others are of relatively recent origin. The principles underlying the major forms of ELISA are outlined, as also the protocols for the production of polyclonal antibodies, and for ELISA. Some DNA-based diagnostics for P. solancearum are also described. Infectivity titration technique to determine virulence of P. solanacearum, and several inoculation techniques to evaluate resistance to groundnut bacterial wilt area also described. The principles underlying each technique, and the technicalities are explained in detail to enable the nonspecialist to understand them Groundnut1994International Crops Research Institute for the Semi-Arid TropicsMonograph

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