?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.relation=http%3A%2F%2Foar.icrisat.org%2F7729%2F&rft.title=Techniques+for+diagnosis+of+Pseudomonas+solanacearum+and+for+resistance+screening+against+groundnut+bacterial+wilt%3A+training+manual&rft.creator=Mehan%2C+V+K&rft.creator=McDonald%2C+D&rft.subject=Groundnut&rft.description=In+this+manual%2C+we+present+a+comprehensive+range+of+methods+for+isolation%2C+identification%2C+and+detection+of+the+groundnut+bacterial+wilt+pathogen%2C+Pseudomonas+solanacearum.+Emphasis+has+been+placed+on+the+use+ofenzymelinked+immunosorbent+assay+(ELISA)+to+detect+the+bacterium+in+plant+tissues%2C+seed%2C+and+soil.+Some+of+the+techniques+described+have+been+in+use+for+some+time+without+undergoing+any+major+improvements%2C+while+others+are+of+relatively+recent+origin.+The+principles+under+lying+the+major+improvements%2C+while+others+are+of+relatively+recent+origin.+The+principles+underlying+the+major+forms+of+ELISA+are+outlined%2C+as+also+the+protocols+for+the+production+of+polyclonal+antibodies%2C+and+for+ELISA.+Some+DNA-based+diagnostics+for+P.+solancearum+are+also+described.+Infectivity+titration+technique+to+determine+virulence+of+P.+solanacearum%2C+and+several+inoculation+techniques+to+evaluate+resistance+to+groundnut+bacterial+wilt+area+also+described.+The+principles+underlying+each+technique%2C+and+the+technicalities+are+explained+in+detail+to+enable+the+nonspecialist+to+understand+them+&rft.publisher=International+Crops+Research+Institute+for+the+Semi-Arid+Tropics&rft.date=1994&rft.type=Monograph&rft.type=NonPeerReviewed&rft.format=application%2Fpdf&rft.language=en&rft.identifier=http%3A%2F%2Foar.icrisat.org%2F7729%2F1%2FRP%25209131.pdf&rft.identifier=++Mehan%2C+V+K+and+McDonald%2C+D++(1994)+Techniques+for+diagnosis+of+Pseudomonas+solanacearum+and+for+resistance+screening+against+groundnut+bacterial+wilt%3A+training+manual.++Manual.+International+Crops+Research+Institute+for+the+Semi-Arid+Tropics%2C+Patancheru%2C+Hyderabad%2C+Andhra+Pradesh.+++++