eprintid: 7465
rev_number: 14
eprint_status: archive
userid: 128
dir: disk0/00/00/74/65
datestamp: 2014-02-06 05:49:04
lastmod: 2016-05-10 08:40:52
status_changed: 2014-02-06 05:49:04
type: book_section
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
creators_name: Kishore Babu, B
creators_name: Sharma, A
creators_name: Sudini, H
icrisatcreators_name: Kishore Babu, B
icrisatcreators_name: Sudini, H
affiliation: ICRISAT(Patancheru)
affiliation: NBAIM(Kusumaur)
country: India
title: DNA Cloning and Sequencing
ispublished: pub
subjects: s2.13
full_text_status: restricted
abstract: The plasmid DNA is cleaved with an enzyme and joined in vitro to foreign DNA; the resulting recombinant plasmids are then used to transform bacteria. The plasmid vectors must be carefully chosen and processed to minimize the effort required to identify and characterize recombinants. This chapter provides guidelines for preparation of DNA fragment for cloning, transformation into chemically competent host, and selection of positive clones. The write-up will also describe basic methods used in the cloning of PCR amplified rRNA gene into appropriate vector and followed by sequencing.
date: 2013
date_type: published
series: Springer Protocols Handbooks
publisher: Springer Berlin Heidelberg
pagerange: 291-302
pages: 352
refereed: TRUE
isbn: 978-3-642-34410-7
book_title: Analyzing Microbes: Manual of Molecular Biology Techniques
editors_name: Arora, D K
editors_name: Das, S
editors_name: Sukumar, M
related_url_url: http://scholar.google.co.in/scholar?as_q=%22DNA+Cloning+and+Sequencing%22&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&btnG=&hl=en&as_sdt=0%2C5
related_url_type: pub
citation:   Kishore Babu, B and Sharma, A and Sudini, H  (2013) DNA Cloning and Sequencing.   In:  Analyzing Microbes: Manual of Molecular Biology Techniques.   Springer Protocols Handbooks .  Springer Berlin Heidelberg, pp. 291-302.  ISBN 978-3-642-34410-7     
document_url: http://oar.icrisat.org/7465/1/ch19.pdf