eprintid: 7393
rev_number: 10
eprint_status: archive
userid: 30
dir: disk0/00/00/73/93
datestamp: 2014-01-26 09:54:07
lastmod: 2014-01-26 09:54:07
status_changed: 2014-01-26 09:54:07
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@cgiar.org
creators_name: Kumar, S
creators_name: Sharma, R
creators_name: Kumar, V
creators_name: Vyas, G K
creators_name: Rathore, A
creators_id: RAU
creators_id: IARI
icrisatcreators_name: Rathore, A
affiliation: SK Rajasthan Agricultural University(Bikaner)
affiliation: IARI(New Delhi)
affiliation: University of Rajasthan(Jaipur)
affiliation: ICRISAT(Patancheru)
country: India
title: Combining molecular-marker and chemical analysis of Capparis deciduas (Capparaceae) in the Thar Desert of Western Rajasthan (India) 
ispublished: pub
subjects: s2.13
subjects: s2.4
full_text_status: public
note: Authors are thankful to Department of
Biotechnology, Government of India for funding
the project “Characterization of underutilized
species of the Thar Desert
abstract: The Thar Desert, a very inhospitable place, accommodates only plant  species  that survive acute drought, unpredictable precipitation, and those can grow  in  the limited moisture of sandy soils. Capparis decidua is among one of the few plants able to grow well under these conditions. This species is highly exploited and has been naturally taken, as local people use it for various purposes like food, timber and fuel, although, no management or conservation efforts have been established. The present study was conducted in this arid area of Western Rajasthan (India) with the aim to obtain preliminary molecular information about this group of plants. We evaluated diversity among 46 samples of C. decidua using chemical parameters and random amplified polymorphic DNA (RAPD) markers. Fourteen chemical parameters and eight minerals (total 22 variables) of this species fruits were estimated. A total of 14 RAPD primers produced 235 band positions, of which 81.27% were polymorphic. Jaccard’s similarity coefficients for RAPD primers ranged from 0.34 to 0.86 with a mean genetic similarity of 0.50. As per observed coefficient of variation, NDF (Neutral Detergent Fiber) content was found to be the most variable trait followed by starch and soluble carbohydrate. The Manhattan dissimilarity coefficient values for chemical parameters ranged between 0.02-0.31 with an average of 0.092. The present study revealed a very low correlation  (0.01) between chemical parameters and RAPD-based matrices. The low correlation between chemical- and RAPD-based matrices indicated that the two methods were different and highly variable. The chemical-based diversity will assist in selection of nutritionally rich samples for medicinal purpose, while genetic diversity to face natural challenges and find sustainable ways to promote conservation for future use. 
date: 2013
date_type: published
publication: Revista de biologia tropical : the international journal of tropical biology and conservation
volume: 61
number: 1
publisher: Universidad de Costa Rica
pagerange: 311-320
refereed: TRUE
issn: 0034-7744
official_url: http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442013000100026
related_url_url: http://scholar.google.co.in/scholar?as_q=%22+Combining+molecular-marker+and+chemical+analysis+of+Capparis+deciduas+%28Capparaceae%29+in+the+Thar+Desert+of+Western+Rajasthan+%28India%29+%22+&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&
related_url_type: pub
funders: Government of India - Department of Biotechnology 
projects: “Characterization of underutilized species of the Thar Desert”.
citation:   Kumar, S and Sharma, R and Kumar, V and Vyas, G K and Rathore, A  (2013) Combining molecular-marker and chemical analysis of Capparis deciduas (Capparaceae) in the Thar Desert of Western Rajasthan (India).  Revista de biologia tropical : the international journal of tropical biology and conservation, 61 (1).  pp. 311-320.  ISSN 0034-7744     
document_url: http://oar.icrisat.org/7393/1/RBP_IJTB_61_1_311-320_2013.pdf