eprintid: 7119
rev_number: 15
eprint_status: archive
userid: 30
dir: disk0/00/00/71/19
datestamp: 2013-09-24 14:50:14
lastmod: 2016-08-12 08:04:31
status_changed: 2013-09-24 14:50:14
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@cgiar.org
creators_name: Bhatnagar-Panwar, M
creators_name: Bhatnagar-Mathur, P
creators_name: Bhaaskarla, V V
creators_name: Dumbala, S R
creators_name: Sharma, K K
icrisatcreators_name: Bhatnagar-Panwar, M
icrisatcreators_name: Bhatnagar-Mathur, P
icrisatcreators_name: Bhaaskarla, V V
icrisatcreators_name: Dumbala, S R
icrisatcreators_name: Sharma, K K
affiliation: ICRISAT(Patancheru)
country: India
title: Rapid, accurate and routine HPLC method for large-scale screening of pro-vitamin A carotenoids in oilseeds
ispublished: pub
subjects: s1.3
subjects: s2.13
full_text_status: restricted
note: This research was supported by grants from HarvestPlus Challenge Program and the Department of Biotechnology, Government of India. We thank the
Directorate of Oilseed Research, Hyderabad, India and the Peanut Breeding unit of
ICRISAT for providing the seeds for this research. The technical support received from
Mohd. Yousuf, D. Pandary and C. Lakshminarayana is duly acknowledged.
abstract: Plant carotenoids, the precursors of vitamin A display several important biological functions as antioxidants and anti-carcinogens. The oilseed crops, owing to their high oil content, form a good matrix for the bioavailability of β-carotene, thereby providing potential targets for biofortification to combat vitamin A deficiency (VAD). However, the screening and characterization of these crops, that otherwise contain very low levels of pro-vitamin A carotenoids has been difficult owing to their poor recovery and strong binding to the oil matrix. Here, we report a rapid method for high volume HPLC analysis involving the extraction and determination of β-carotene in four oilseed crops (peanut, soybean, sunflower and mustard). This included a comprehensive study of the factors that potentially influence the qualitative and quantitative yields of β-carotene in these crops. This is the first crop-independent HPLC method for the quantification of pro-vitamin A carotenoids that shows excellent recovery and reproducibility (>90 percentage recovery in oil) using small tissue sample and is capable of processing up to 30 samples per day. The protocol is sensitive, and enables better detection and separation of individual carotenoids by reducing artefacts during extraction, purification and chromatography that can be used for routine screening of oilseeds.
date: 2015
date_type: published
publication: Journal of Plant Biochemistry and Biotechnology
volume: 24
number: 1
publisher: Springer India
pagerange: 84-92
id_number: 10.1007/s13562-013-0239-1
refereed: TRUE
issn: 0974-1275
official_url: http://dx.doi.org/10.1007/s13562-013-0239-1
related_url_url: http://scholar.google.co.in/scholar?as_q=%22Rapid%2C+accurate+and+routine+HPLC+method+for+large-scale+screening+of+pro-vitamin+A+carotenoids+in+oilseeds%22&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&btnG=&hl=en&as_sdt
related_url_type: pub
funders: Government of India - Department of Biotechnology
projects: HarvestPlus Challenge Program
citation:   Bhatnagar-Panwar, M and Bhatnagar-Mathur, P and Bhaaskarla, V V and Dumbala, S R and Sharma, K K  (2015) Rapid, accurate and routine HPLC method for large-scale screening of pro-vitamin A carotenoids in oilseeds.  Journal of Plant Biochemistry and Biotechnology, 24 (1).  pp. 84-92.  ISSN 0974-1275     
document_url: http://oar.icrisat.org/7119/1/JPBB_Bhatnagar_etal._Rapid-post-print_2013.pdf
document_url: http://oar.icrisat.org/7119/7/art%253A10.1007%252Fs13562-013-0239-1.pdf