eprintid: 7104
rev_number: 15
eprint_status: archive
userid: 19
dir: disk0/00/00/71/04
datestamp: 2013-09-17 10:49:49
lastmod: 2014-08-06 10:24:18
status_changed: 2014-08-06 10:24:18
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
creators_name: Chakradhar, T
creators_name: Reddy, B P
creators_name: Srinivas, N
creators_name: Reddy, P C O
icrisatcreators_name: Chakradhar, T
icrisatcreators_name: Reddy, B P
icrisatcreators_name: Srinivas, N
affiliation: ICRISAT(Patancheru)
affiliation: Y.V. University(Kadapa)
country: India
title: Identification and validation of an allele specific marker associated with pungency in Capsicum spp.
ispublished: pub
subjects: s2
full_text_status: restricted
keywords: Capsicum spp. Marker assisted selection, Pun1, Capsaicinoids, Pungency, Allele
abstract: Pungency or heat in Capsicum spp. is due to the accumulation of unique secondary compounds known as capsaicinoids in their placental tissues. Detecting presence or absence of pungency at the nursery stage is a challenging task in CMS based hybrid pepper breeding programs. In this study a DNA sequence possibly related to pungency trait with high similarity to Pun1 or At3 gene was investigated. Nucleotide alignment of the obtained sequences and corresponding fragment from the data base has revealed a 16bp deletion in C.annuum ‘Maor’. A multiplex agarose based co-dominant marker was designed to detect the identified polymorphism and named it as Cen1. This Cen1 marker is validated in a panel of 27 pepper genotypes belonging to C.annuum, C.chinensis, C.frutescens and C.baccatum for its wide utility. All these Capsicum accessions were correctly discriminated with phenotype. In addition, the ability of Cen1 marker to discriminate homozygous and heterozygous plants was demonstrated in F1 hybrids crossed from a non pungent ‘Maor’ and a pungent ‘Habanero’. The Cen1 marker was also associated with phenotypic character in the tested genotypes. Moreover the linkage association of Cen1 with At3 or Pun1 gene has also been discussed. Therefore the developed functional marker in this study will be highly useful in marker assisted selection (MAS) programmes, germplasam characterization and seed purity testing of chilli
date: 2013
date_type: published
publication: Current Trends in Biotechnology and Pharmacy
volume: 7
number: 1
publisher: Association of Biotechnology and Pharmacy
pagerange: 544-551
refereed: TRUE
issn: 2230-7303
official_url: http://indianjournals.com/ijor.aspx?target=ijor:ctbp&volume=7&issue=1&article=008
related_url_url: http://scholar.google.co.in/scholar?as_q=Identification+and+Validation+of+an+Allele+Specific+Marker+Associated+with+Pungency+in+Capsicum+spp.&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&btnG=&hl=en&as_sdt=0%2C5
related_url_type: pub
citation:   Chakradhar, T and Reddy, B P and Srinivas, N and Reddy, P C O  (2013) Identification and validation of an allele specific marker associated with pungency in Capsicum spp.  Current Trends in Biotechnology and Pharmacy, 7 (1).  pp. 544-551.  ISSN 2230-7303     
document_url: http://oar.icrisat.org/7104/1/Current%20Trends%20in%20Biotechnology%20and%20Pharmacy_7_1_544-551_2013.pdf