TY  - JOUR
N2  - A fast neutron (FN)-mutagenised population was generated in Pisum sativum L. (pea) to enable the identification and isolation of genes underlying traits and processes. Studies of several phenotypic traits have clearly demonstrated the utility of the resource by associating gene deletions with phenotype followed by functional tests exploiting additional mutant sources, from both induced and natural variant germplasm. For forward genetic screens, next generation sequencing methodologies provide an opportunity for identifying genes associated with deletions rapidly and systematically. The application of rapid reverse genetic screens of the fast neutron mutant pea population supports conclusions on the frequency of deletions based on phenotype alone. These studies also suggest that large deletions affecting one or more loci can be non-deleterious to the pea genome, yielding mutants that could not be obtained by other means. Deletion mutants affecting genes associated with seed metabolism and storage are providing unique opportunities to identify the products of complex and related gene families, and to study the downstream consequences of such deletions.
N1  - This paper originates from a presentation at the ?VI International Conference on Legume Genetics and Genomics (ICLGG)?
Hyderabad, India, 2?7 October 2012.
AV  - public
KW  - fast neutron mutagenesis
KW  -  genomic deletions
KW  -  seed proteins.
A1  - Domoney, C
A1  - Knox, M
A1  - Moreau , C
A1  - Ambrose, M
A1  - Palmer, J M
A1  - Smith, P
A1  - Christodoulou, V
A1  - Isaac, P G
A1  - Hegarty, M
A1  - Blackmore, T
A1  - Swain, M
A1  - Ellis, N
TI  - Exploiting a fast neutron mutant genetic resource in Pisum sativum (pea) for functional genomics
UR  - http://dx.doi.org/10.1071/FP13147
JF  - Functional Plant Biology
SP  - A
Y1  - 2013///
ID  - icrisat7078
EP  - J
SN  - 1445-4408
PB  - CSIRO Publishing
ER  -