OAR@ICRISATPeanut clump virus and Polymyxa graminis interactions with pearl millet (Pennisetum glaucum [L.] R. Br.) and Sorghum(Sorghum bicolor [L.] Moench)BDieryckauthorPDelfosseauthorGOttoauthorXSauvenierauthorCBragardauthorALegreveauthorThe susceptibility of 6 pearl millet (Ankoutess, ICMV IS 89305, ICMV IS 92222, ICMV IS 94206, Sadoré local and Zatib) and 6 sorghum (Irat 204, Mota Maradi, Nad 1, Sariaso 10, Sepon 82 and Zinder local) accessions to Peanut clump virus and its vector (Polymyxa graminis) was evaluated during the rainy season of 2002 under field conditions in Sadoré, Niger. The frequencies of infection of roots and leaves, analysed by a penicillinase-based DAS-ELISA, confirmed the differences among the pearl millet and sorghum accessions. In pearl millet, the virus was detected in roots and leaves, whereas in sorghum, infection was common in roots but rare in leaves. This was linked with the seed transmission of PCV in millet and not in sorghum. These results confirmed preliminary observations on the movement of the virus within these plant species, underlining the potential role of the viral P15 gene, a suppressor of post-transcriptional gene inactivation. Susceptibility to P. graminis f.sp. tropicalis was assessed under controlled conditions using a zoospore inoculation assay. Infection was quantified by a real-time quantitative PCR assay targeting the ribosomial DNA, and highlighting the differences between plant accessions and species. In addition to guidance on the selection of resistant cultivars, this study also sought to provide a toolbox for improving the understanding of the plant/vector/virus interactionsGroundnut2005Association pour les Études et Recherches de Zoologie appliquée et de PhytopathologieArticle

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