OAR@ICRISATA Technique to Screen for Resistance to Stem Rot caused by Sclerotium rolfsii in Groundnut under Greenhouse conditionsSPandeauthorR JNarayanaauthorM VReddyauthorDMcDonaldauthorA technique was standardized to screen for resistance to stem rot (Sclerotium rolfsii Sacc.) in groundnut (Arachis hypogaea L.). The technique involved inoculation of 10 day old groundnut plants raised in pots by spreading mycelial propagules of 5. rolfsii grown on sorghum grain medium (SGM) on soil surface, and covering them with groundnut leaf debris collected form the field at the time of harvesting of the previous season's crop. Inoculated plants were placed in a greenhouse in which temperatures ranged between 16° C and 34°C. Pots were watered at 24 h intervals to ensure high soil moisture. Almost 100% stem rot was produced on a known susceptible cultivar Robut 33-1 within 10–12 days after inoculation. Stem rot developed at all plant growth stages (10 to 90 days), but disease development was slower in older (>40 day-old) plants than in younger plants. Time between inoculation and stem rot expression also increased with an increase in the age of plants. None of the 350 groundnut genotypes screened using the greenhouse technique showed resistance when the inoculated plants were incubated at 23–36° C but eleven of them showed less than 20% incidence at 16–31° C.GroundnutPlant Pathology1994Plant Protection Association of IndiaArticle

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