eprintid: 6467
rev_number: 15
eprint_status: archive
userid: 19
dir: disk0/00/00/64/67
datestamp: 2013-01-25 04:51:56
lastmod: 2014-02-07 05:10:39
status_changed: 2013-01-25 04:51:56
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
creators_name: Srinivasa Rao, P
creators_name: Kumar, P S
creators_name: Sonti, R V
icrisatcreators_name: Srinivasa Rao, P
affiliation: ICRISAT(Patancheru)
affiliation: Centre for Cellular and Molecular Biology(Hyderabad)
affiliation: Nuziveedu Seeds(Hyderabad)
country: India
title: Excised radicle tips as a source of genomic DNA for PCR-based genotyping and melting curve analysis in cotton
ispublished: pub
subjects: s2.13
full_text_status: restricted
keywords: Cotton; cry1Ac; genomic DNA isolation; high-resolution melting curve analysis; radicle tip; seed purity testing
abstract: Genomic DNA isolation in cotton is complicated because of the presence of secondary metabolites that are inhibitory to PCR amplification. We report here that radicle tips, but not other parts of cotton seedlings, yield high-quality DNA that is readily amenable for PCR. The radicle-tip-excised seedlings retain viability because of the formation of adventitious roots. We demonstrate the utility of this method in distinguishing homozygotes from heterozygotes in a cotton breeding population and in hybrid seed purity testing
date: 2013
date_type: published
publication: Journal of Biosciences
volume: 38
number: 1
publisher: Springer
pagerange: 167-172
refereed: TRUE
issn: 0250-5991
official_url: http://dx.doi.org/10.1007/s12038-012-9285-4
related_url_url: http://scholar.google.co.in/scholar?as_q=Excised+radicle+tips+as+a+source+of+genomic+DNA+for+PCR-based+genotyping+and+melting+curve+analysis+in+cotton&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&btnG=&hl=en&as_sdt=0%2C
related_url_type: pub
funders: Government of India - Department of Biotechnology 
citation:   Srinivasa Rao, P and Kumar, P S and Sonti, R V  (2013) Excised radicle tips as a source of genomic DNA for PCR-based genotyping and melting curve analysis in cotton.  Journal of Biosciences, 38 (1).  pp. 167-172.  ISSN 0250-5991     
document_url: http://oar.icrisat.org/6467/1/JournalofBiosciences_38_1_167-172_2013.pdf