TY  - JOUR
AV  - restricted
A1  - Srinivasa Rao, P
A1  - Kumar, P S
A1  - Sonti, R V
TI  - Excised radicle tips as a source of genomic DNA for PCR-based genotyping and melting curve analysis in cotton
UR  - http://dx.doi.org/10.1007/s12038-012-9285-4
JF  - Journal of Biosciences
SN  - 0250-5991
PB  - Springer
N2  - Genomic DNA isolation in cotton is complicated because of the presence of secondary metabolites that are inhibitory to PCR amplification. We report here that radicle tips, but not other parts of cotton seedlings, yield high-quality DNA that is readily amenable for PCR. The radicle-tip-excised seedlings retain viability because of the formation of adventitious roots. We demonstrate the utility of this method in distinguishing homozygotes from heterozygotes in a cotton breeding population and in hybrid seed purity testing
KW  - Cotton; cry1Ac; genomic DNA isolation; high-resolution melting curve analysis; radicle tip; seed purity testing
Y1  - 2013///
SP  - 167
ID  - icrisat6467
EP  - 172
VL  - 38
IS  - 1
ER  -