@article{icrisat6426,
           title = {Optimizing seed quality during germplasm regeneration in pearl millet},
       publisher = {Kluwer Academic},
          author = {N K Rao and P J Bramel and K N Reddy and S D Singh and A G Girish and S A Rao and V Mahalakshmi},
           pages = {153--157},
            year = {2002},
          volume = {49},
         journal = {Genetic Resources and Crop Evolution},
          number = {2},
        keywords = {Germplasm regeneration; Pearl millet; Pollination control;
Seed longevity; Water stress},
             url = {http://oar.icrisat.org/6426/},
        abstract = {The effect of water stress during flowering and grain filling on seed longevity was studied in three pearl millet genotypes,ICTP-8202, ICTP-8203 and MBH-110. The seeds were produced by three pollination methods; open pollination, selfing(individual panicles enclosed in paper bags), and cluster bagging (panicles from 3?4 adjacent plants enclosed in a paper bag), stored in air-tight plastic bottles underambient conditions (20?40 ?C,30?80\%RH) and germination was tested at 12-month'sintervals. The seeds lost germination completely after six years ofstorage in all treatments. Analysis of variance of the estimates of potential seed longevity (i.e. the seed lot constantK i of the seed viabilityequation) showed significant effects of water stress andpollination method (P {\ensuremath{<}} 0.01). The interaction between irrigation treatment and method of pollination control was also significant (P {\ensuremath{<}} 0.05). Averaged over genotypes and pollination methods,potential longevity was greatest(K i = 2.8) in theirrigated control, and averaged over genotypes and irrigationtreatments, it was greatest (K i= 3.1) in seeds produced by open pollination. Theimplications of these results were discussed in relation to germplasmseed production}
}