relation: http://oar.icrisat.org/6406/ title: Identification of the Sex Pheromone of Holotrichia reynaudi creator: Ward, A creator: Moore, C creator: Anitha, V creator: Wightman, J creator: John Roge, D subject: Entomology description: The male attractant pheromone of the scarab beetle Holotrichia reynaudi, an agricultural pest native to southern India, was extracted from abdominal glands of females with hexane and analyzed by gas chromatography–mass spectrometry. Field testing of the candidate chemicals, indole, phenol, and anisole, both alone and as binary mixtures, led us to conclude that anisole was the major component of the sex pheromone. Neither male nor female beetles were attracted to indole or phenol on their own. Similarly, when indole and anisole were combined, the attractiveness of the solution did not increase over that obtained with anisole alone. However, combination of phenol and anisole did alter the attractiveness of anisole, with fewer male beetles attracted to the binary mixture than to anisole on its own. The behavior of female beetles was not altered by any of the chemicals tested. Anisole is also the sex pheromone of H. consanguinea, making this the first known example of two melolonthine scarabs sharing the same pheromone publisher: Kluwer Academic date: 2002 type: Article type: PeerReviewed format: application/pdf language: en identifier: http://oar.icrisat.org/6406/1/JournalofChemicalEcology_28_3_515-522_2002.pdf identifier: Ward, A and Moore, C and Anitha, V and Wightman, J and John Roge, D (2002) Identification of the Sex Pheromone of Holotrichia reynaudi. Journal of Chemical Ecology, 28 (3). pp. 515-522. ISSN 0098-0331 relation: http://dx.doi.org/10.1007/s10705-007-9141-3