OAR@ICRISATGenomic sequence derived simple sequence repeats markers. A case study with medicago spp.VMahalakshmiauthorPAparnaauthorSRamadeviauthorROrtizauthorSimple sequence repeats (SSR) or microsatellites are becoming standard DNA markers for plant genome analysis and are being used as markers in marker assisted breeding. De novo generation of microsatellite markers through laboratory-based screening of SSR-enriched genomic libraries is highly time consuming and expensive. An alternative is to screen the public databases of related model species where abundant sequence data is already available. All the genomic sequences of Medicago from the public domain database were searched and analysed for di, tri and tetra nucleotide repeats. Of the total 156 000 sequences which were searched, 7325 sequences contained repeat motif and may yield SSR which will yield product sizes of around 200 bp. Of these, the most abundantly found repeats were the trinucleotide (5210) group. Except for a very small proportion (436), these link to the gene annotation database at TIGR (http://www.tigr.org). To facilitate further exploration of this resource, a dynamic database with options to search and link to other resources is available at (http://www.icrisat.org/text/research/ grep/homepage/genomics/medssrs1.asp) and on CDs from V.Mahalakshmi@cgiar.org Genetics and Genomics2002Universidad Católica de Valparaíso - Chile Article

For work being deposited by its own author: In self-archiving this collection of files and associated bibliographic metadata, I grant OAR@ICRISAT the right to store them and to make them permanently available publicly for free on-line. I declare that this material is my own intellectual property and I understand that OAR@ICRISAT does not assume any responsibility if there is any breach of copyright in distributing these files or metadata. (All authors are urged to prominently assert their copyright on the title page of their work.)

For work being deposited by someone other than its author: I hereby declare that the collection of files and associated bibliographic metadata that I am archiving at OAR@ICRISAT) is in the public domain. If this is not the case, I accept full responsibility for any breach of copyright that distributing these files or metadata may entail.

Clicking on the deposit button indicates your agreement to these terms.