eprintid: 6158
rev_number: 13
eprint_status: archive
userid: 30
dir: disk0/00/00/61/58
datestamp: 2012-10-01 03:01:12
lastmod: 2014-01-25 13:12:12
status_changed: 2012-10-01 03:01:12
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@cgiar.org
creators_name: Prasad, K
creators_name: Bhatnagar-Mathur, P
creators_name: Waliyar, F
creators_name: Sharma, K K
icrisatcreators_name: Prasad, K
icrisatcreators_name: Bhatnagar-Mathur, P
icrisatcreators_name: Waliyar, F
icrisatcreators_name: Sharma, K K
affiliation: ICRISAT(Patancheru)
country: India
title: Overexpression of a chitinase gene in transgenic peanut confers enhanced resistance to major soil borne and foliar fungal pathogens
ispublished: pub
subjects: s1.3
subjects: s2.13
full_text_status: restricted
keywords: Arachis hypogaea  –  Aspergillus flavus  – Groundnut – Rice chitinase – Transgenic – Peanut – Late leaf spot disease – Rust
note: We thank Dr. S. Muthukrishnan, Department of
Biochemistry, Kansas State University, for kindly providing the rice
chitinase gene. We also thank R. Kanaka Reddy for excellent technical
help.
abstract: A chitinase gene from rice (Rchit) was introduced into three varieties of peanut through Agrobacterium-mediated genetic transformation resulting in 30 transgenic events harboring the Rchit gene. Stable integration and expression of the transgenes were confirmed using PCR, RT-PCR and Southern blot analysis. Progeny derived from selfing of the primary transgenic events revealed a Mendelian inheritance pattern (3:1) for the transgenes. The chitinase activity in the leaves of the transgenic events was 2 to 14-fold greater than that in the non-transformed control plants. Seeds of most transgenic events showed 0–10 % A. flavus infection during in vitro seed inoculation bioassays. Transgenic peanut plants evaluated for resistance against late leaf spot (LLS) and rust using detached leaf assays showed longer incubation, latent period and lower infection frequencies when compared to their non-transformed counterparts. A significant negative correlation existed between the chitinase activity and the frequency of infection to the three tested pathogens. Three progenies from two transgenic events displayed significantly higher disease resistance for LLS, rust and A. flavus infection and are being advanced for further evaluations under confined field conditions to confirm as sources to develop peanut varieties with enhanced resistance to these fungal pathogens.
date: 2013
date_type: published
publication: Journal of Plant Biochemistry and Biotechnology
volume: 22
number: 2
publisher: Springer India
pagerange: 222-233
refereed: TRUE
issn: 0974-1275
official_url: http://dx.doi.org/10.1007/s13562-012-0155-9
related_url_url: http://scholar.google.co.in/scholar?as_q=%22Overexpression+of+a+chitinase+gene+in+transgenic+peanut+confers+enhanced+resistance+to+major+soil+borne+and+foliar+fungal+pathogens%22&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&
related_url_type: pub
citation:   Prasad, K and Bhatnagar-Mathur, P and Waliyar, F and Sharma, K K  (2013) Overexpression of a chitinase gene in transgenic peanut confers enhanced resistance to major soil borne and foliar fungal pathogens.  Journal of Plant Biochemistry and Biotechnology, 22 (2).  pp. 222-233.  ISSN 0974-1275     
document_url: http://oar.icrisat.org/6158/7/GNRicechitinase-Kalynaietal.pdf
document_url: http://oar.icrisat.org/6158/1/2012Kalyani_etal_JPBB_overexpression.pdf