eprintid: 60
rev_number: 14
eprint_status: archive
userid: 1
dir: disk0/00/00/00/60
datestamp: 2011-06-01 08:43:45
lastmod: 2011-06-10 09:17:39
status_changed: 2011-06-01 08:43:45
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@cgiar.org
item_issues_count: 0
creators_name: Gowda, M V C
creators_name: Bhat, R S
creators_name: Sujay, V
creators_name: Kusuma, P
creators_name: Kumari, Varsha
creators_name: Varshney, R K
creators_name: Bhat, S
icrisatcreators_name: Varshney, R K
affiliation: University of Agricultural Sciences(Dharwad)
affiliation: ICRISAT(Patancheru)
affiliation: CIMMYT(Mexico)
country: India
title: Characterization of AhMITE1 transposition and its association with the mutational and
evolutionary origin of botanical types in peanut (Arachis spp.)
ispublished: pub
subjects: s1.3
full_text_status: restricted
keywords: AhMITE1;Transposition activation;Peanut; Allopolyploidy;Botanical types
agrotags: <b>Agrotags</b>     - groundnuts | mutants | genomes | planting | genetics | transposons | crops | developmental stages | brassica oleracea botrytis | social groups <br><b>Fishtags</b>    - NOT-AVAILABLE<br><b>Geopoliticaltags</b> - brazil | india | maine | virginia | argentina | peru | bolivia | usa | mexico | uruguay
note: The authors are grateful to Dr H.D. Upadhyaya,
ICRISAT, for providing the wild peanut species, and to Mr Ravi
Koppulu, ICRISAT, for technical help.
abstract: AhMITE1 is an active miniature inverted
repeat transposable element (MITE) in peanut (Arachis
hypogaea L). Its transpositional activity from a particular
(FST1-linked) site within the peanut genome was checked
using AhMITE1-specifc PCR, which used a forward primer
annealing to the 50-flanking sequence and a reverse primer
binding to AhMITE1. It was found that transposition activation
was induced by stresses such as ethyl methane
sulfonate (EMS), gamma irradiation, environmental conditions,
and tissue culture. Excision and insertion of
AhMITE1 at this particular site among the mutants led to
gross morphological changes resembling alternate subspecies
or botanical types. Analysis of South American landraces
revealed the presence of AhMITE1 at the site among
most of the spp. fastigiata types, whereas the element was
predominantly missing from spp. hypogaea types, indicating
its strong association. Four accessions of the primitive
allotetraploid, A. monticola were devoid of AhMITE1
at the site, indicating only recent activation of the element,
possibly because of the ‘‘genomic shock’’ resulting from
hybridization followed by allopolyploidization.
date: 2011
date_type: published
publication: Plant Systematics and Evolution
volume: 291
number: 3-4
publisher: Springer
pagerange: 153-158
refereed: TRUE
official_url: http://dx.doi.org/10.1007/s00606-010-0373-3
related_url_url: http://scholar.google.co.in/scholar?as_q=%22Characterization+of+AhMITE1+transposition+and+its+association+with+the+mutational+and+evolutionary+origin+of+botanical+types+in+peanut+%28Arachis+spp.%29%22&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_oc
related_url_type: author
citation:   Gowda, M V C and Bhat, R S and Sujay, V and Kusuma, P and Kumari, Varsha and Varshney, R K and Bhat, S  (2011) Characterization of AhMITE1 transposition and its association with the mutational and evolutionary origin of botanical types in peanut (Arachis spp.).  Plant Systematics and Evolution, 291 (3-4).  pp. 153-158.      
document_url: http://oar.icrisat.org/60/1/1311_merged.pdf
document_url: http://oar.icrisat.org/60/2/1311_published.pdf