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        <dc:title>Characterization of AhMITE1 transposition and its association with the mutational and&#13;
evolutionary origin of botanical types in peanut (Arachis spp.)</dc:title>
        <dc:creator>Gowda, M V C</dc:creator>
        <dc:creator>Bhat, R S</dc:creator>
        <dc:creator>Sujay, V</dc:creator>
        <dc:creator>Kusuma, P</dc:creator>
        <dc:creator>Kumari, Varsha</dc:creator>
        <dc:creator>Varshney, R K</dc:creator>
        <dc:creator>Bhat, S</dc:creator>
        <dc:subject>Groundnut</dc:subject>
        <dc:description>AhMITE1 is an active miniature inverted&#13;
repeat transposable element (MITE) in peanut (Arachis&#13;
hypogaea L). Its transpositional activity from a particular&#13;
(FST1-linked) site within the peanut genome was checked&#13;
using AhMITE1-specifc PCR, which used a forward primer&#13;
annealing to the 50-flanking sequence and a reverse primer&#13;
binding to AhMITE1. It was found that transposition activation&#13;
was induced by stresses such as ethyl methane&#13;
sulfonate (EMS), gamma irradiation, environmental conditions,&#13;
and tissue culture. Excision and insertion of&#13;
AhMITE1 at this particular site among the mutants led to&#13;
gross morphological changes resembling alternate subspecies&#13;
or botanical types. Analysis of South American landraces&#13;
revealed the presence of AhMITE1 at the site among&#13;
most of the spp. fastigiata types, whereas the element was&#13;
predominantly missing from spp. hypogaea types, indicating&#13;
its strong association. Four accessions of the primitive&#13;
allotetraploid, A. monticola were devoid of AhMITE1&#13;
at the site, indicating only recent activation of the element,&#13;
possibly because of the ‘‘genomic shock’’ resulting from&#13;
hybridization followed by allopolyploidization.</dc:description>
        <dc:publisher>Springer</dc:publisher>
        <dc:date>2011</dc:date>
        <dc:type>Article</dc:type>
        <dc:type>PeerReviewed</dc:type>
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        <dc:language>en</dc:language>
        <dc:identifier>http://oar.icrisat.org/60/1/1311_merged.pdf</dc:identifier>
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        <dc:language>en</dc:language>
        <dc:identifier>http://oar.icrisat.org/60/2/1311_published.pdf</dc:identifier>
        <dc:identifier>  Gowda, M V C and Bhat, R S and Sujay, V and Kusuma, P and Kumari, Varsha and Varshney, R K and Bhat, S  (2011) Characterization of AhMITE1 transposition and its association with the mutational and evolutionary origin of botanical types in peanut (Arachis spp.).  Plant Systematics and Evolution, 291 (3-4).  pp. 153-158.      </dc:identifier>
        <dc:relation>http://dx.doi.org/10.1007/s00606-010-0373-3</dc:relation></oai_dc:dc>
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