eprintid: 5958
rev_number: 10
eprint_status: archive
userid: 19
dir: disk0/00/00/59/58
datestamp: 2012-06-07 05:31:11
lastmod: 2013-09-20 03:48:08
status_changed: 2012-06-07 05:31:11
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
creators_name: Anitha, S
creators_name: Waliyar, F
creators_name: Reddy, A S
creators_name: Rao, R
creators_name: Rao, Raghunadha
creators_name: Kumar, P L
icrisatcreators_name: Anitha, S
icrisatcreators_name: Waliyar, F
icrisatcreators_name: Reddy, A S
icrisatcreators_name: Kumar, P L
affiliation: ICRISAT(Patancheru)
affiliation: Department of Microbiology and Immunoserology(Hyderabad)
affiliation: Nizam’s Institute of Medical Sciences(Hyderabad)
affiliation: IITA(Ibadan)
country: India
country: Nigeria
title: Development of a simple enzyme-linked immunosorbent assay for quantitative estimation of aflatoxin B1 albumin adduct in humans
ispublished: pub
subjects: s2
full_text_status: public
abstract: In the present study, high titred polyclonal antibodies were produced against synthetic AFB1-lys and developed a simple indirect competitive (IC)-ELISA for quantitative estimation of AFB1-lys adducts in human serum albumin. IC-ELISA was validated by testing 250 blood samples that include 85 HBV positive samples from unidentified subjects from the Apollo Health City, Hyderabad, and 165 blood samples collected from ICRISAT campus, Hyderabad, India. All the samples that were positive to AFB1-lys were from HBV-positive subjects, indicating a potential risk of hepatocellular carcinoma in 4.8% of the subjects tested in the present study. This validation confirms the suitability of IC-ELISA, which is simple, cost-effective and enables high-throughput analysis
date: 2011
date_type: published
publication: Current Science
volume: 101
number: 7
publisher: Indian Academy of Sciences
pagerange: 844-846
refereed: TRUE
issn: 0011-3891
official_url: http://cs-test.ias.ac.in/cs/Volumes/101/07/0844.pdf
related_url_url: http://scholar.google.co.in/scholar?as_q=Development+of+a+simple+enzyme-linked+immunosorbent+assay+for+quantitative+estimation+of+aflatoxin+B1+albumin+adduct+in+humans&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&hl=en&
related_url_type: pub
citation:   Anitha, S and Waliyar, F and Reddy, A S and Rao, R and Rao, Raghunadha and Kumar, P L  (2011) Development of a simple enzyme-linked immunosorbent assay for quantitative estimation of aflatoxin B1 albumin adduct in humans.  Current Science, 101 (7).  pp. 844-846.  ISSN 0011-3891     
document_url: http://oar.icrisat.org/5958/1/CurrentScience_101_7_844-846%20_2011.pdf