TY  - JOUR
AV  - restricted
A1  - Bohorova, N
A1  - Zhang, W
A1  - Julstrum, P
A1  - McLean, S
A1  - Luna, M
A1  - Brito, R M
A1  - Diaz, L
A1  - Ramos, M E
A1  - Estanol, P
A1  - Pacheco, M
A1  - Salgado, M
A1  - Hoisington, D A
TI  - Production of transgenic tropical maize with cryIAb and cryIAc genes via microprojectile bombardment of immature embryos 
UR  - http://dx.doi.org/10.1007/s001220051255 
JF  - TAG Theoretical and Applied Genetics
SN  - 1432-2242
PB  - Springer Verlag
N1  - D. A. Hoisington is presently with ICRISAT (Patancheru)
N2  - To enhance the level of resistance to insects in
tropical maize germplasm we have developed techniques
to successfully transform elite tropical maize inbred
based on the activity of specific cryI proteins against
four major maize pests ? corn earworm, fall armyworm,
southwestern corn borer and sugarcane borer. Constructs
containing cryIAb or cryIAc synthetic genes were used.
To generate transgenic plants we have established methods
for biolistic bombardment and the selection and regeneration
of immature embryos and calli from the elite
tropical lines CML72, CML216, CML323, CML327 and
hybrids. Transgenic plants resistant to the herbicide BastaTM
contained the bands for the cry, bar and gus genes
as detected by Southern blot analyses. A simple leaf bioassay
presented varying levels of resistance to Southwestern
corn borer of transgenic tropical maize carrying
the cryIAc gene. Analyses of the progenies confirmed
the sexual transmission of the introduced genes and their
stable expression
KW  - Tropical maize · Transformation ·
Plant regeneration · Insect resistance ·
Bacillus thuringiensis (Bt)
Y1  - 1999///
SP  - 437
ID  - icrisat5743
EP  - 444
VL  - 99
IS  - 3-4
ER  -