http://oar.icrisat.org/id/eprint/5743 2012-03-16T06:55:46Z info:eu-repo/semantics/descriptiveMetadata http://oar.icrisat.org/5743/ Production of transgenic tropical maize with cryIAb and cryIAc genes via microprojectile bombardment of immature embryos Bohorova, N Zhang, W Julstrum, P McLean, S Luna, M Brito, R M Diaz, L Ramos, M E Estanol, P Pacheco, M Salgado, M Hoisington, D A Agriculture-Farming, Production, Technology, Economics Maize To enhance the level of resistance to insects in tropical maize germplasm we have developed techniques to successfully transform elite tropical maize inbred based on the activity of specific cryI proteins against four major maize pests – corn earworm, fall armyworm, southwestern corn borer and sugarcane borer. Constructs containing cryIAb or cryIAc synthetic genes were used. To generate transgenic plants we have established methods for biolistic bombardment and the selection and regeneration of immature embryos and calli from the elite tropical lines CML72, CML216, CML323, CML327 and hybrids. Transgenic plants resistant to the herbicide BastaTM contained the bands for the cry, bar and gus genes as detected by Southern blot analyses. A simple leaf bioassay presented varying levels of resistance to Southwestern corn borer of transgenic tropical maize carrying the cryIAc gene. Analyses of the progenies confirmed the sexual transmission of the introduced genes and their stable expression Springer Verlag 1999 Article PeerReviewed application/pdf en http://oar.icrisat.org/5743/1/TheorApp%20Gene_99_437%E2%80%93444_1999.pdf Bohorova, N and Zhang, W and Julstrum, P and McLean, S and Luna, M and Brito, R M and Diaz, L and Ramos, M E and Estanol, P and Pacheco, M and Salgado, M and Hoisington, D A (1999) Production of transgenic tropical maize with cryIAb and cryIAc genes via microprojectile bombardment of immature embryos. TAG Theoretical and Applied Genetics, 99 (3-4). pp. 437-444. ISSN 1432-2242 http://dx.doi.org/10.1007/s001220051255 info:eu-repo/semantics/objectFile info:eu-repo/semantics/humanStartPage