eprintid: 5719
rev_number: 7
eprint_status: archive
userid: 30
dir: disk0/00/00/57/19
datestamp: 2012-03-15 07:29:33
lastmod: 2012-03-15 07:29:33
status_changed: 2012-03-15 07:29:33
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@cgiar.org
creators_name: Pellegrineschi, A
creators_name: et al, .
icrisatcreators_name: Hoisington, D A
affiliation: CIMMYT(Mexico)
affiliation: ICRISAT(Patancheru)
country: Mexico
country: India
title: Transgenic wheat plants: a powerful breeding source 
ispublished: pub
subjects: s2.3
full_text_status: restricted
note: D. A. Hoisington is presently with ICRISAT (Patancheru)
abstract: Plant breeders are always interested in new genetic resources. In the past, the sources have been limited to existing
germplasm. Genetic engineering now provides the opportunity for almost unlimited strategies to create novel
resources. As a first stage, the Applied Biotechnology Center (ABC) at CIMMYT developed a method for the mass
production of fertile transgenic wheat (Triticum aestivum L.) that yields plants ready for transfer to soil in 13–14
weeks after the initiation of cultures, and, over the course of a year, an average production of 5–6 transgenic plants
per day. CIMMYT elite cultivars are co-bombarded with marker gene and a gene of interest with co-transformation
efficiencies around 25–30%. The reliability of this method opens the possibility for the routine introduction of
novel genes that may induce resistance to diseases and abiotic stresses, allow the modification of dough quality,
and increase the levels of micronutrients such as iron, zinc, and vitamins. The first group of genes being evaluated
by the ABC are the pathogenesis related (PR) proteins, such as the thaumatin-like protein (TLP) from barley,
chitinase, and 1–3 β-glucanase. Stable integration of the genes in the genome and inheritance in the progeny were
determined by phenotypical analyses that challenged the plants against a wide range of pathogens. Using these
genes, we have recovered more than 1200 independent events (confirmed by PCR and Southern blot analyses)
that show responses to the pathogens that range from tolerance to hypersensitive reactions. The quantity and antifungal
activity of the endogenous thaumatin-like proteins were analyzed in T 1 and T2 progeny plants.Western blot
analyses showed different protein patterns of the wheat endogenous TLPs. Preliminary results indicated that some
patterns increased the resistance of transgenic wheat plants to Alternaria triticina. This relationship is being further
investigated.
date: 2001
date_type: published
publication: Euphytica
volume: 119
number: 1-2
publisher: Springer
pagerange: 135-138
refereed: TRUE
issn: 1573-5060
official_url: http://dx.doi.org/10.1023/A:1017573817633 
related_url_url: http://scholar.google.co.in/scholar?hl=en&q=allintitle%3A+%22Transgenic+wheat+plants%3A+a+powerful+breeding+source%22&btnG=Search&as_sdt=0%2C5&as_ylo=&as_vis=0
related_url_type: author
citation:   Pellegrineschi, A and et al, .  (2001) Transgenic wheat plants: a powerful breeding source.  Euphytica, 119 (1-2).  pp. 135-138.  ISSN 1573-5060     
document_url: http://oar.icrisat.org/5719/1/Euphytica_119_133%E2%80%93136_2001.pdf